"
Team:CU-Boulder/Notebook/Protocols
From 2013.igem.org
(Difference between revisions)
| Line 107: | Line 107: | ||
<div class="shadow-bottom"> | <div class="shadow-bottom"> | ||
<dl> | <dl> | ||
| - | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ProteinPurificationAgaroseGel">Protein Purification | + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ProteinPurificationAgaroseGel">Protein Purification Via Agarose Gel</a> <a href="https://static.igem.org/mediawiki/2013/6/61/ProteinPurificationAgaroseGel.pdf"><i class="icon-download"></i></a></dt> |
<dd>This protocol covers how to extract proteins from cells and purify it.</dd> | <dd>This protocol covers how to extract proteins from cells and purify it.</dd> | ||
</dl> | </dl> | ||
Latest revision as of 00:57, 26 September 2013
- Embedding PDF Files
- In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.
- Embedding Google Docs
- Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.
- Chemically Competent Cells
- This protocol details how to prepare chemically competent cells.
Related Experiments
- Chemical Transformations
- In this protocol we cover how to use our chemically competent cells to perform transformations.
Related Experiments
- Plasmid Minipreps
- Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.
Related Experiments
- Restriction (Double) Digests
- Our method of performing restriction double digests is detailed in this protocol.
- Gel Purification of DNA
- Here we detail a fast and simple method of purifying DNA from a gel.
- Ligation Reactions
- This protocol covers how we set up out ligation reactions.
- Homemade Buffer Compositions
- The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.
Related Experiments
- Recycling DNA Mini-columns
- This protocol covers how to recycle mini-columns used in plasmid miniprep systems.
Related Experiments
- Protein Miniprep
- This protocol covers how to extract proteins from cells.
- Protein Purification Via Agarose Gel
- This protocol covers how to extract proteins from cells and purify it.
