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Team:Calgary/Notebook/Protocols/VerificationDigest
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<li>0.2µL enzyme 1</li> | <li>0.2µL enzyme 1</li> | ||
<li>0.2µL enzyme 2</li> | <li>0.2µL enzyme 2</li> | ||
| - | <li>3.6µL | + | <li>3.6µL ddH<sub>2</sub>O (deionized water)</li> |
</ul> | </ul> | ||
<li>Incubate at 37 degrees Celsius for 1h</li> | <li>Incubate at 37 degrees Celsius for 1h</li> | ||
Revision as of 23:16, 27 September 2013
Verification Digest
Verification Digest
Reagents and Materials
- Restriction enzymes
- Appropriate buffer
- Plasmid purified DNA
- 1% agarose gel
Protocol
- In a tube, add (per reaction):
- 4µL Plasmid purified DNA
- 1µL appropriate buffer
- 0.2µL enzyme 1
- 0.2µL enzyme 2
- 3.6µL ddH2O (deionized water)
- Incubate at 37 degrees Celsius for 1h
- Run 10µL in a 1% agarose gel at 100V
