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Team:Calgary/Project/OurSensor/Detector
From 2013.igem.org
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<h1>Detector</h1> | <h1>Detector</h1> | ||
| - | <p><b>T</b>ranscription <b>A</b>ctivator-<b>L</b>ike <b>E</b>ffectors (TALEs) are naturally occurring proteins that bind to DNA. These proteins are found in <i>Xanthomonas</i> species, a plant pathogen, and originally serve as a pathogenic factor. Because TALEs have a highly conserved modular region for DNA binding, they are particularly amenable to genetic engineering and customization | + | <p><b>T</b>ranscription <b>A</b>ctivator-<b>L</b>ike <b>E</b>ffectors (TALEs) are naturally occurring proteins that bind to DNA. These proteins are found in <i>Xanthomonas</i> species, a plant pathogen, and originally serve as a pathogenic factor. Because TALEs have a highly conserved modular region for DNA binding, they are particularly amenable to genetic engineering and customization.</p> |
<p>For proof of concept, we worked with TALEs from the Parts Registry.</p> | <p>For proof of concept, we worked with TALEs from the Parts Registry.</p> | ||
<p>For our detecting system itself, we have engineered TALE proteins that binds to two different regions of the Shiga Toxin II gene, <i>Stx2</i>, found in <i>E. coli</i> and other entero-haemorrhagic bacteria.</p> | <p>For our detecting system itself, we have engineered TALE proteins that binds to two different regions of the Shiga Toxin II gene, <i>Stx2</i>, found in <i>E. coli</i> and other entero-haemorrhagic bacteria.</p> | ||
Revision as of 19:23, 24 September 2013
Detector
Detector
Transcription Activator-Like Effectors (TALEs) are naturally occurring proteins that bind to DNA. These proteins are found in Xanthomonas species, a plant pathogen, and originally serve as a pathogenic factor. Because TALEs have a highly conserved modular region for DNA binding, they are particularly amenable to genetic engineering and customization.
For proof of concept, we worked with TALEs from the Parts Registry.
For our detecting system itself, we have engineered TALE proteins that binds to two different regions of the Shiga Toxin II gene, Stx2, found in E. coli and other entero-haemorrhagic bacteria.
