Team:Bielefeld-Germany/Labjournal/Analytics
From 2013.igem.org
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<h1 style="color:#ff6600;">Analytics</h1> | <h1 style="color:#ff6600;">Analytics</h1> | ||
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- | <a name="analytics"><span style="color:#ff6600; padding-left=22px">[ | + | <a name="analytics"><span style="color:#ff6600; padding-left=22px">[SDS PAGE]</span></a> |
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<div class="analytics" > | <div class="analytics" > | ||
- | + | <br><b><u> subtitle</u></b> | |
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+ | <a name="tecan"><span style="color:#ff6600; padding-left=22px">[Fluorescence measurements]</span></a> | ||
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+ | <br><b><u>Fluorescence measurements </u></b> | ||
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+ | Measuring of mRFP with Tecan Infinite® M200 platereader | ||
+ | Take at least 500 µL sample for each measurement (200 µL is needed for one measurement) so you can perform a repeat determination | ||
+ | Freeze biological samples at -80 °C for storage, keep cell-free at 4 °C in the dark | ||
+ | To measure the samples thaw at room temperature and fill 200 µL of each sample in one well of a black, flat bottom 96 well microtiter plate (perform at least a repeat determination) | ||
+ | Measure the fluorescence in a platereader (we used a Tecan Infinite® M200 platereader) with following settings: | ||
+ | 20 sec orbital shaking (1 mm amplitude with a frequency of 87.6 rpm) | ||
+ | Measurement mode: Top | ||
+ | Excitation: 584 nm | ||
+ | Emission: 620 nm | ||
+ | Number of reads: 25 | ||
+ | Manual gain: 100 | ||
+ | Integration time: 20 µs | ||
+ | <br> | ||
<br><b><u>subtitle</u></b> | <br><b><u>subtitle</u></b> | ||
Revision as of 19:42, 25 September 2013
Analytics
subtitle
Fluorescence measurements Measuring of mRFP with Tecan Infinite® M200 platereader Take at least 500 µL sample for each measurement (200 µL is needed for one measurement) so you can perform a repeat determination Freeze biological samples at -80 °C for storage, keep cell-free at 4 °C in the dark To measure the samples thaw at room temperature and fill 200 µL of each sample in one well of a black, flat bottom 96 well microtiter plate (perform at least a repeat determination) Measure the fluorescence in a platereader (we used a Tecan Infinite® M200 platereader) with following settings: 20 sec orbital shaking (1 mm amplitude with a frequency of 87.6 rpm) Measurement mode: Top Excitation: 584 nm Emission: 620 nm Number of reads: 25 Manual gain: 100 Integration time: 20 µs
subtitle