In the iGEM, most teams have inserted their Biobrick parts or devices into plasmids and have used them. It is true that there are many good points to use plasmids, for instance, it is easy to do transformation, and it is convenient to use high copy plasmid when you want to get high amount of gene expression. However, there are some bad points to use plasmids too. For example, when the reproduction starting point of plural plasmids are covered, they can’t be put in E.coli at the same time. Also it is difficult to control closely expression of the genes which are in .plasmids.

Therefore, in this year, our team tried to establish and standardize a new method to insert Biobrick parts or devices in a genome of E.coli and use them. Also, according to this method, we really inserted the device which we designed in a genome of E.coli and functionalized it.