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Team:OUC-China/Microfluidics
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| - | <div class="span9"><p style="font-weight:normal;"><font size="2px"> Magnetospirillum | + | <div class="span9"><p style="font-weight:normal;"><font size="2px"> detecting the magnetism of small amount of bacterial sample is always difficult, because not only the sample is so little that normal magnetic spectrophotometer could not fetch precise data, but also the debugging of magnetic spectrophotometer is quite difficult and expensive for the exist of earth magnetic field.<br /> Since our compartment gene is from Magnetospirillum Magneticum which is able to do directional movement along the geomagnetic field due to its magnetosome, so we need to culture the Magnetospirillum Magneticum AMB-1 strain firstly. While we cultured the AMB-1 bacteria strain, we found that if we hope to evaluate whether our culture scheme is feasible, we should confilm not only the grow, but also the magnetism of the bacteria is normal .So we designed an easy solution of bacteria magnetism detection for smallamount of samplesto make our bacteria magnetism detection easier and cheaper. When we need to detect the bacteria sample, we could just use Microfluidic chip in our own lad instead of processing our sample to TEM detection waiting for a long time and wasting so much money.<br /> According to our experiment,We utilize the fact that the Magnetospirillum Magneticum has magnetism to build a mathematical model reflecting the relationship between the bacterial number and magnetic field intensity. So we design the microfluidic chip below to compose the detection.</font></p> |
| - | <img src="https://static.igem.org/mediawiki/2013/b/b1/Ouc-Microfluidics.jpg" height="500" width="600" /><br />Fig.1 Structure of microfluidic chip. | + | <img src="https://static.igem.org/mediawiki/2013/b/b1/Ouc-Microfluidics.jpg" height="500" width="600" /><br />Fig.1 Structure of microfluidic chip.<br /><br /> |
| + | <div class="span9"><p style="font-weight:normal;"><font size="2px"> Just as the figure above, the microfluidic chip is divided into three channels, Channel 1,Channel 2 and Channel 3. The Channel 1 is a narrow channel allows the Bacterial to broth slowly flows through, then the bacteria enterChannel 2, a relatively wide area, also calledthe magnetic deflection area, in whichbacteria will change course under the action of magnetic field we apply. After deflected in the magnetic field, the bacteria will go into Channel 3 divided into fiftysmaller channels with a filter and the bacteria will stay inside under the effect of the filter.After a certain amount of time, we count the bacteria in the small channel under the microscope.Finallywe build a model according the relationship between bacterial number and magnetic field intensity to show the magnetism of bacteria sample.</font></p></div> | ||
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Revision as of 16:22, 26 September 2013
Microfluidics
detecting the magnetism of small amount of bacterial sample is always difficult, because not only the sample is so little that normal magnetic spectrophotometer could not fetch precise data, but also the debugging of magnetic spectrophotometer is quite difficult and expensive for the exist of earth magnetic field.
Since our compartment gene is from Magnetospirillum Magneticum which is able to do directional movement along the geomagnetic field due to its magnetosome, so we need to culture the Magnetospirillum Magneticum AMB-1 strain firstly. While we cultured the AMB-1 bacteria strain, we found that if we hope to evaluate whether our culture scheme is feasible, we should confilm not only the grow, but also the magnetism of the bacteria is normal .So we designed an easy solution of bacteria magnetism detection for smallamount of samplesto make our bacteria magnetism detection easier and cheaper. When we need to detect the bacteria sample, we could just use Microfluidic chip in our own lad instead of processing our sample to TEM detection waiting for a long time and wasting so much money.
According to our experiment,We utilize the fact that the Magnetospirillum Magneticum has magnetism to build a mathematical model reflecting the relationship between the bacterial number and magnetic field intensity. So we design the microfluidic chip below to compose the detection.
Fig.1 Structure of microfluidic chip.
Just as the figure above, the microfluidic chip is divided into three channels, Channel 1,Channel 2 and Channel 3. The Channel 1 is a narrow channel allows the Bacterial to broth slowly flows through, then the bacteria enterChannel 2, a relatively wide area, also calledthe magnetic deflection area, in whichbacteria will change course under the action of magnetic field we apply. After deflected in the magnetic field, the bacteria will go into Channel 3 divided into fiftysmaller channels with a filter and the bacteria will stay inside under the effect of the filter.After a certain amount of time, we count the bacteria in the small channel under the microscope.Finallywe build a model according the relationship between bacterial number and magnetic field intensity to show the magnetism of bacteria sample.
