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Team:Freiburg/Project/modeling
From 2013.igem.org
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<title>CrispR</title> | <title>CrispR</title> | ||
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<script type="text/javascript"> | <script type="text/javascript"> | ||
$(document).ready( function () { | $(document).ready( function () { | ||
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var TestVar = form.Sequenz.value; | var TestVar = form.Sequenz.value; | ||
var length = TestVar.length; | var length = TestVar.length; | ||
Revision as of 15:29, 26 September 2013
crRNA-Design
This tool helps you to design a crRNA-insert for pX334a. The oligos contain overhangs which fit to the BbsI-overhangs by pX334a.
For repression of gene transcription by targeting the coding sequence
it´s crucial to target the non template (= coding) DNA strand.
Therefore the oligos must be designed as
follows:
- Search at your desired target sequenz for a CCN (reverse complement of the PAM sequence) at the coding strand.
- Extract the following (3') 30 nucleotides.
- Extract the reverse complement.
- Add AAAC at the 5' end and GT at the 3' end. This will be your fist oligo.
- Take the sequence from step 2 and add TAAAAC at the 5' end. This will be your second oligo.
