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Team:Calgary/Notebook/Protocols/PrussianBlueFerritinSynthesis
From 2013.igem.org
(Difference between revisions)
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<h1>Prussian Blue Ferritin Synthesis</h1> | <h1>Prussian Blue Ferritin Synthesis</h1> | ||
<h2> Reagents and Materials </h2> | <h2> Reagents and Materials </h2> | ||
| + | <ul> | ||
<li>Horse Spleen Ferritin (47mg/mL) </li> | <li>Horse Spleen Ferritin (47mg/mL) </li> | ||
<li>Hydrogen Chloride (pH 3.0)</li> | <li>Hydrogen Chloride (pH 3.0)</li> | ||
<li> K<sub>4</sub>Fe(CN)<sub>6</sub> (0.025M) </li> | <li> K<sub>4</sub>Fe(CN)<sub>6</sub> (0.025M) </li> | ||
<li> Sodium acetate-acetic acid buffer (pH 3.6)</li> | <li> Sodium acetate-acetic acid buffer (pH 3.6)</li> | ||
| + | </ul> | ||
| + | <br> | ||
<p> 20µL of horsespleen ferritin (47mg/mL) was added to 20mL of HCl (pH 3.0) and 150µL of K<sub>4</sub>Fe(CN)<sub>6</sub> (0.025M). The solution was kept under 4℃ for 12 hours while constantly being stirred.</p> | <p> 20µL of horsespleen ferritin (47mg/mL) was added to 20mL of HCl (pH 3.0) and 150µL of K<sub>4</sub>Fe(CN)<sub>6</sub> (0.025M). The solution was kept under 4℃ for 12 hours while constantly being stirred.</p> | ||
Revision as of 17:16, 26 September 2013
Synthesis
Prussian Blue Ferritin Synthesis
Reagents and Materials
- Horse Spleen Ferritin (47mg/mL)
- Hydrogen Chloride (pH 3.0)
- K4Fe(CN)6 (0.025M)
- Sodium acetate-acetic acid buffer (pH 3.6)
20µL of horsespleen ferritin (47mg/mL) was added to 20mL of HCl (pH 3.0) and 150µL of K4Fe(CN)6 (0.025M). The solution was kept under 4℃ for 12 hours while constantly being stirred.
After the 12 hour period, 5mL of the solution was dialyzed against 500mL of sodium acetate-acetic acid buffer (pH 3.6) for 24 hours, with the buffer being replaced after 8 hours.
