Team:Bielefeld-Germany/Labjournal/May
From 2013.igem.org
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*GldA PCR product was isolated by Agarose gel electrophorese and purificated. | *GldA PCR product was isolated by Agarose gel electrophorese and purificated. | ||
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- | [[Image:IGEM_Bielefeld_GldA_PCR_Gel.jpg| | + | [[Image:IGEM_Bielefeld_GldA_PCR_Gel.jpg|right|thumb|200px|'''Figure 1:''' Agarosegel from PCR on the GldA gene of ''Escherichia coli'' KRX strain with forward and reverse primer GldA. For Ladder we used GeneRuler™ 1 kb DNA Ladder fromThermo Scientific.]] |
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Revision as of 13:18, 27 September 2013
May
Milesstones
1.Week
Organization
- We were already able to find sponsors for our team: IIT BIEKUBA, IIT Biotech, Evonik, PlasmidFactory and FisherScientific will support us.
MFC
Mediators
Glycerol dehydrogenase
- Primerdesign for isolation of GldA gene from Escherichia coli KRX strain, with overlaps for Biobrick Prefix and Suffix:
- Forward Primer GldA (43 bp): GAATTCGCGGCCGCTTCTAGATGGACCGCATTATTCAATCACC
- Reverse Primer GldA (45 bp): CTGCAGCGGCCGCTACTAGTATTATTCCCACTCTTGCAGGAAACG
Cytochromes
Biosafety
Porines
2.Week
Organization
- Distribution of our team in various subgroups for best work efficiency.
- We’ve organized a working group barbecue to thank the AG of Dr. Jörn Kalinowski in the CeBiTec for the appropriation of space and support in the laboratory.
MFC
Mediators
Cytochromes
Biosafety
Porines
3.Week
Organization
- Planning the trip to the European jamboree in Lyon from 11.-13. October 2013.
MFC
Mediators
Glycerol dehydrogenase
- Starting first cultivation of Escherichia coli KRX strain for complete genome isolation.
- Successful genome isolation of Escherichia coli KRX.
- Successful PCR on the GldA gene of Escherichia coli KRX strain.
- GldA PCR product was isolated by Agarose gel electrophorese and purificated.
Cytochromes
Biosafety
Porines
4.Week
Organization
- We will also take part at the congress "Next generation of biotechnological processes 2020+" in Berlin at 27. June 2013.
- Having a second presentation at Merck KGaA head office in Darmstadt for explaining our project in detail. We are happy that Merck will be our next supporter in 2013.
MFC
Mediators
Cytochromes
Biosafety
Porines
Contents |