Team:TU-Munich/Notebook/Materials

From 2013.igem.org

(Difference between revisions)
(→Oligonucleotides)
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|xylE_a834_rev
|xylE_a834_rev
|<code>5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3'</code><br>
|<code>5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3'</code><br>
 +
|-
 +
|
 +
|p Act_for
 +
|<code>5'- AAGCTTTCTAGAGTCGACGAGGTCATTCATATG - 3'</code><br>
 +
|-
 +
|
 +
|pAct_rev
 +
|<code>5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGCTTCTACCTACAAAAAAGCTC - 3'</code><br>
 +
|-
 +
|
 +
|t35S_for
 +
|<code>5'- AAGCTTTCTAGAGCTAGAGTCCGCAAAAATCACC - 3'</code><br>
 +
|-
 +
|
 +
|t35S_rev
 +
|<code>5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGGTCACTGGATTTTGGTTTTAGG - 3'</code><br>
 +
|-
 +
|
 +
|Npt_for
 +
|<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGATCGGATCCTGTCAAACAC - 3'</code><br>
 +
|-
 +
|
 +
|Npt_rev
 +
|<code>5'- CGGCCGCTACTAGTAATCAAGCTTGACAGGAGGC - 3'</code><br>
 +
|-
 +
|
 +
|miniMCSII_fw
 +
|<code>5'- CTAGACAATTGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'</code><br>
 +
|-
 +
|
 +
|miniMCSII_rv
 +
|<code>5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCAATTGT - 3'</code><br>
 +
|-
 +
|
 +
|
 +
|<code>5'-  - 3'</code><br>
|-
|-
|
|

Revision as of 12:10, 12 August 2013



Reagents

Chemicals

Reagent Supplier

Kits and Standards

Plastic Disposables

Oligonucleotides

Name Sequence
EreA_for 5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘
EreA-rev 5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘
EreA_SDMI_for 5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘
EreA_SDMI_rev 5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3'
EreA_SDMII_for 5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3'
EreA_SDMII_rev 5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3'
EreA_SDMIII_for 5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3'
EreA_SDMIII_rev 5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3'
EreB_for 5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3'
EreB-rev 5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3'
EreB_SDMI_for 5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3'
EreB_SDMI_rev 5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3'
EreB_SDMII_for 5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3'
EreB_SDMII_rev 5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3'
EreB_SDMIII_for 5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3'
EreB_SDMIII_rev 5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3'
TEV_fw 5'- gcgcgtctagatggccggcggagaaagcttgtttaaggggc - 3'
TEV_rv 5'- atataaccggtattcatgagttgagtcgcttcc - 3'
TEV_t387c_fw 5'- atggtgtcagacactagctgcacattcccttcatc - 3'
TEV_t387c_rv 5'- gatgaagggaatgtgcagctagtgtctgacaccat - 3'
N_split_TEV_fw 5'- attcgcggccgcttctag - 3'
N_split_TEV_rv 5'- ttcgaaaccggtagtttggaagttggttgtcac - 3'
C_split_TEV_fw 5'- aagctttctagatggccggcaagagcatgtctagcatggtg - 3'
C_split_TEV_rv 5'- ccgctactagtattaaccgg - 3'
PIF6_2-100_fw 5'- aagctttctagatggccggcatgttcttaccaaccgattattgttgc - 3'
PIF6_2-100_rv 5'- ttcgaaaccggtgtcaacatgtttattgctttc - 3'
MinMCS_fo 5'- ctagacctaggaagcttcctgcaggactagtaagcttctgca - 3'
MinMCS_rev 5'- gaagcttactagtcctgcaggaagcttcctaggt - 3'
Bpu_for 5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3'
Bpu_rev 5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3'
Bpu_SDMI_for 5'- cacttaagcattttctacgggtcgatcacaccattca - 3'
Bpu_SDMI_rev 5'- tgaatggtgtgatcgacccgtagaaaatgcttaagtg - 3'
Bpu_SDMII_fo 5'- gacacgattaaatgtgtatgcgggcttagctggattttatttg - 3'
Bpu_SDMII_re 5'- caaataaaatccagctaagcccgcatacacatttaatcgtgtc - 3'
Bpu_SDMIII_fo 5'- cacccttaaaaataaagctggctgcggacaggaag - 3'
Bpu_SDMIII_re 5'- cttcctgtccgcagccagctttatttttaagggtg - 3'
Bpu_SDMIV_fo 5'- cgtacgctcactcttacgggtacacaggataaata - 3'
Bpu_SDMIV_re 5'- tatttatcctgtgtacccgtaagagtgagcgtacg - 3'
xylE_for 5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG - 3'
xylE_rev 5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC - 3'
xylE_c312a_for 5'- GAACAGTTGTGGACGGCGCGTGCGC - 3'
xylE_c312a_rev 5'- GCGCACGCGCCGTCCACAACTGTTC - 3'
xylE_g483a_for 5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3'
xylE_g483a_rev 5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3'
xylE_a834g_for 5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3'
xylE_a834_rev 5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3'
p Act_for 5'- AAGCTTTCTAGAGTCGACGAGGTCATTCATATG - 3'
pAct_rev 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGCTTCTACCTACAAAAAAGCTC - 3'
t35S_for 5'- AAGCTTTCTAGAGCTAGAGTCCGCAAAAATCACC - 3'
t35S_rev 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGGTCACTGGATTTTGGTTTTAGG - 3'
Npt_for 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGATCGGATCCTGTCAAACAC - 3'
Npt_rev 5'- CGGCCGCTACTAGTAATCAAGCTTGACAGGAGGC - 3'
miniMCSII_fw 5'- CTAGACAATTGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'
miniMCSII_rv 5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCAATTGT - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'
5'- - 3'

Instruments

Electrophoresis

Photometers

Nanodrop Spectrohpotometer

Moss-cultivation

References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.


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