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Team:Bielefeld-Germany/Labjournal/June
From 2013.igem.org
(Difference between revisions)
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====Mediators==== | ====Mediators==== | ||
| + | =====Glycerol dehydrogenase===== | ||
| + | *Isolation of shipping vector pSB1C3 out of 2013 Distribution Kit Plate 5 Well 3A with insert Part RFP (J04450) for better transformation characterization (Distribution Kit BioBrick isolation) | ||
| + | *Transformation of BBa_J04450 into ''Escherichia coli'' KRX strain. | ||
| + | *Plasmid isolation of BBa_J04450. | ||
| Line 115: | Line 119: | ||
====Organization==== | ====Organization==== | ||
| - | *Nothing organizational | + | *Nothing organizational. |
====MFC==== | ====MFC==== | ||
Revision as of 13:35, 27 September 2013
June
Milesstones
5.Week
Organization
- iGEM-Team Bielefeld will support the ‘CeBiTec Pupil Academy’ from 26.-30. August with an own experiment.
MFC
Mediators
Cytochromes
Biosafety
Porines
6.Week
Organization
- Thanks to NEB Biolabs for the free iGEM kit with many useful laboratory things for all German iGEM teams.
- We are working on our first press release.
- Having a short radio contribution in the Bielefeld university campus radio (radio 87.9 hertz).
MFC
Mediators
Glycerol dehydrogenase
- Isolation of shipping vector pSB1C3 out of 2013 Distribution Kit Plate 5 Well 3A with insert Part RFP (J04450) for better transformation characterization (Distribution Kit BioBrick isolation)
- Transformation of BBa_J04450 into Escherichia coli KRX strain.
- Plasmid isolation of BBa_J04450.
Cytochromes
Biosafety
Porines
7.Week
Organization
- Nothing organizational.
MFC
Mediators
Cytochromes
- Cultivation of Shewanella oneidensis MR-1 in liquid LB medium at 30 °C
- Isolation of genomic DNA from S. oneidensis
- 4-2006-451: Conc.:453.1 ng/µl OD260/280: 1.92 OD260/230:2.78
- 4-2006-452: Conc.:447.1 ng/µl OD260/280: 1.92 OD260/230:2.67
- Dilution to PCR template: 4-2006-453: 5.5ng/µl
- Amplification of the mtrCAB cluster
- Gradient: 55.8-56.7-57.8-59.1-60.4-61.7-62.9-63.9
- Clear Bands at the expected 5.2kb
- PCR-CleanUp
- PCR-cleanup of No.2 and No.5 with Macherey-Nagel-Kit and NanoDrop-Measurement
- Lane2: 4-2106-451: 7.4 ng/µl
- Lane5: 4-2106-451: 8.5 ng/µl
Biosafety
Porines
8.Week
Organization
- Let’s go to Lyon, flights are booked for the European jamboree in Lyon from 11.-13. October 2013.
- We will participate at ‘BioNRW pHD Student Convention’ in Düsseldorf at 13. July.
MFC
Mediators
Cytochromes
Biosafety
Porines
Contents |
