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Team:TMU-Tokyo/Parts
From 2013.igem.org
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| - | <li><a href="http://parts.igem.org/Part:BBa_K1015013"> | + | <li><a href="http://parts.igem.org/Part:BBa_K1015013">BBa_K1015013</a>：<b>Km-Blunt end </b><br> |
This is what connected the blunt end to cohesive end of pSB1C3.</li> | This is what connected the blunt end to cohesive end of pSB1C3.</li> | ||
<span style="line-height:50%"><Br></span> | <span style="line-height:50%"><Br></span> | ||
| - | <li><a href="http://parts.igem.org/Part:BBa_K1015014"> | + | <li><a href="http://parts.igem.org/Part:BBa_K1015014">BBa_K1015014</a>：<b>IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR)</b><br> |
This device produce FLP and AadA(streptomycin resistant gene) . But if site specific recombination be caused between IRR site and IRL site by FimE recombinase, pTet promoter change "OFF" state and FLP production stop. | This device produce FLP and AadA(streptomycin resistant gene) . But if site specific recombination be caused between IRR site and IRL site by FimE recombinase, pTet promoter change "OFF" state and FLP production stop. | ||
</li> | </li> | ||
<span style="line-height:50%"><Br></span> | <span style="line-height:50%"><Br></span> | ||
| - | <li><a href="http://parts.igem.org/Part:BBa_K1015015"> | + | <li><a href="http://parts.igem.org/Part:BBa_K1015015">BBa_K1015015</a>：<b>araC-pBAD-hbiF-Tc(genome insertion parts between araC and araA)</b><br> |
With bacteriophage λ recombination system, this device insert into Escherichia coli genome. This device produce hbiF recombinase and tetracycline resistance protein (tetA). HbiF production level are regulated by arabinose inducible promoter pBAD.</li> | With bacteriophage λ recombination system, this device insert into Escherichia coli genome. This device produce hbiF recombinase and tetracycline resistance protein (tetA). HbiF production level are regulated by arabinose inducible promoter pBAD.</li> | ||
<span style="line-height:50%"><Br></span> | <span style="line-height:50%"><Br></span> | ||
| - | <li><a href="http://parts.igem.org/Part:BBa_K1015016"> | + | <li><a href="http://parts.igem.org/Part:BBa_K1015016">BBa_K1015016</a>：<b>lacI+FRT+Cm</b><br> |
This is genome insertion part. The device is: R(SaApR)homology sequence(BBa_K1015018) + lacI(BBa_K1015008) + FRT(BBa_K1015009) + mhpA homology sequence(BBa_K1015009). | This is genome insertion part. The device is: R(SaApR)homology sequence(BBa_K1015018) + lacI(BBa_K1015008) + FRT(BBa_K1015009) + mhpA homology sequence(BBa_K1015009). | ||
</li> | </li> | ||
<span style="line-height:50%"><Br></span> | <span style="line-height:50%"><Br></span> | ||
| - | <li><a href="http://parts.igem.org/Part:BBa_K1015017">BBa_K1015017 | + | <li><a href="http://parts.igem.org/Part:BBa_K1015017">BBa_K1015017</a>：<b>FRT+Ap(for RED system)</b><br> |
This is genome insertion parts. Site specific recombination site. if it happens by Flp sequences between 2 place of FRT is left out. </li> | This is genome insertion parts. Site specific recombination site. if it happens by Flp sequences between 2 place of FRT is left out. </li> | ||
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Revision as of 17:25, 27 September 2013
Submitted parts
Parts data
- BBa_K1015013：Km-Blunt end
This is what connected the blunt end to cohesive end of pSB1C3. - BBa_K1015014：IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR)
This device produce FLP and AadA(streptomycin resistant gene) . But if site specific recombination be caused between IRR site and IRL site by FimE recombinase, pTet promoter change "OFF" state and FLP production stop. - BBa_K1015015：araC-pBAD-hbiF-Tc(genome insertion parts between araC and araA)
With bacteriophage λ recombination system, this device insert into Escherichia coli genome. This device produce hbiF recombinase and tetracycline resistance protein (tetA). HbiF production level are regulated by arabinose inducible promoter pBAD. - BBa_K1015016：lacI+FRT+Cm
This is genome insertion part. The device is: R(SaApR)homology sequence(BBa_K1015018) + lacI(BBa_K1015008) + FRT(BBa_K1015009) + mhpA homology sequence(BBa_K1015009). - BBa_K1015017：FRT+Ap(for RED system)
This is genome insertion parts. Site specific recombination site. if it happens by Flp sequences between 2 place of FRT is left out.
