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Team:TMU-Tokyo/Notebook experiment/1st
From 2013.igem.org
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DNA Purification following fragment from gel.<Br> | DNA Purification following fragment from gel.<Br> | ||
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| - | <Tr><Td>PtetR1 | + | <Tr><Td>PtetR1, FLP, Sm, araC-P, hbiF, Tc, lacI, FRT-Cm, FRT-Ap, Km-Blunt</Td></Tr> |
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<A href="https://2013.igem.org/Team:TMU-Tokyo/Notebook_experiment/2st">>>Go to next week</A> | <A href="https://2013.igem.org/Team:TMU-Tokyo/Notebook_experiment/2st">>>Go to next week</A> | ||
Revision as of 16:15, 27 September 2013
Experiment > Parts Synthesis > 1st week
September 2nd
PCR following fragment.
| Fragment | PtetR1 | PtetR2 | FLP | Sm | araC-P |
| Primer | CmNR-stu FLP-IRR/IRL | CmCR-stu FLP-IRR/IRL | FLP-L FLP-R | FLP-SaApL CmC | acaC-2 BAD-C3 |
| Templete | IRR/IRL-GFP (BBa_K880002) | IRR/IRL-GFP (BBa_K880002) | pFTGT | m(C2-Ap)Sm | pBAD/Myc-HisA |
| Fragment | hbiF | Tc | lacI | FRT-Cm | FRT-Ap |
| Primer | pBAD-HbiF RTcN-hbiF | TcN TcC3 | 141-70 FRT-lacIC | R6Kori-Xho-3R R(FRT)2 | lacI-R(FRT)2 mhpA-SaApR |
| Templete | hbiF (BBa_K880000) | Escherichia coli MG1655 recA⁻ (cell suspention) | Escherichia coli MG1655 genome | pSG76C | pSG76A2 |
Result
1: PterR1, 2: PtetR2, 3: FLP, 4: Sm, 5: araC-P,
6: hbiF, 7: Tc, 8: LacI, 9: FRT-Cm, 10: FRT-Ap
September 3rd
Vector Remodeling with PCR. Insertion Recognition sites (Blunt).
| Fragment | Km-Blunt |
| Primer | Km-Blunt-N Km-Blunt-C |
| Templete | pACYC177 |
Result
DNA Purification following fragment from gel.
| PtetR1, FLP, Sm, araC-P, hbiF, Tc, lacI, FRT-Cm, FRT-Ap, Km-Blunt |
>>Go to next week
