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Team:TMU-Tokyo/Notebook experiment/4th
From 2013.igem.org
(Difference between revisions)
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1: pSB1C3-Km, 2-4: pSB1A3-Km, 5-6: fimE-pBAD<Br> | 1: pSB1C3-Km, 2-4: pSB1A3-Km, 5-6: fimE-pBAD<Br> | ||
<Br> | <Br> | ||
| + | <Br> | ||
| + | Check PCR.<Br> | ||
| + | <div style="text-align:center;"> | ||
| + | <Table cellpadding="5"> | ||
| + | <Tr> | ||
| + | <Td>Fragment</Td><Td>(control)</Td><Td>Fragment 3 - ApCm</Td> | ||
| + | </Tr> | ||
| + | <Tr> | ||
| + | <Td>Primer</Td><Td>141-49<Br>SaApL</Td><Td>141-49<Br>SaApL</Td> | ||
| + | </Tr> | ||
| + | <Tr> | ||
| + | <Td>Templete</Td><Td><i>Escherichia coli</i><Br>MG1655 red<Br>(Cell suspention)</Td><Td>(Cell suspention)</Td> | ||
| + | </Tr> | ||
| + | </Table> | ||
| + | </div> | ||
| + | Result.<Br> | ||
| + | <IMG Src="https://static.igem.org/mediawiki/2013/b/b5/TMU-Tokyo_0922_1.jpg"><Br> | ||
| + | 1-9: Fragment 3 - ApCm<Br> | ||
<Br> | <Br> | ||
| + | Check PCR.<Br> | ||
| + | <div style="text-align:center;"> | ||
| + | <Table cellpadding="5"> | ||
| + | <Tr> | ||
| + | <Td>Fragment</Td><Td>(control)</Td><Td>Fragment 3 - ApCm</Td><Td>Fragment 3 - ApCm</Td> | ||
| + | </Tr> | ||
| + | <Tr> | ||
| + | <Td>Primer</Td><Td>2 sets of the others</Td><Td>141-1<Br>141-20</Td><Td>141-1<Br>141-74</Td> | ||
| + | </Tr> | ||
| + | <Tr> | ||
| + | <Td>Templete</Td><Td><i>Escherichia coli</i><Br>MG1655 red<Br>(Cell suspention)</Td><Td>(Cell suspention)</Td> | ||
| + | </Tr> | ||
| + | </Table> | ||
| + | </div> | ||
| + | Result.<Br> | ||
| + | <IMG Src="https://static.igem.org/mediawiki/2013/9/90/TMU-Tokyo_0922_2.jpg"><Br> | ||
| + | 1-7: 141-1 and 141-20, 8: control(141-1 and 141-20),<Br> | ||
| + | 9-15: 141-1 and 141-74, 16: control(141-1 and 141-74),<Br> | ||
| + | <Br> | ||
| + | <Br> | ||
| + | |||
| + | Digestion following plasmid.<Br> | ||
| + | <Table><Tr><Td>pSB1C3-Km-Blunt</Td></Tr></Table> | ||
| + | <Br> | ||
| + | <Br> | ||
| + | P1 Preparation following fragment.<Br> | ||
| + | <Table><Tr><Td>Fragment 1, 2</Td></Tr></Table> | ||
| + | <Br> | ||
| + | <Br> | ||
<b>September 23th</b><Br> | <b>September 23th</b><Br> | ||
<Br> | <Br> | ||
Revision as of 22:18, 27 September 2013
Experiment > 4th week
September 22th
Mini prep following plasmid.
| fimE-pBAD(in pSB1A3), pSB1C3-Km, pSB1A3-Km |
1: pSB1C3-Km, 2-4: pSB1A3-Km, 5-6: fimE-pBAD
Check PCR.
| Fragment | (control) | Fragment 3 - ApCm |
| Primer | 141-49 SaApL | 141-49 SaApL |
| Templete | Escherichia coli MG1655 red (Cell suspention) | (Cell suspention) |
1-9: Fragment 3 - ApCm
Check PCR.
| Fragment | (control) | Fragment 3 - ApCm | Fragment 3 - ApCm |
| Primer | 2 sets of the others | 141-1 141-20 | 141-1 141-74 |
| Templete | Escherichia coli MG1655 red (Cell suspention) | (Cell suspention) |
1-7: 141-1 and 141-20, 8: control(141-1 and 141-20),
9-15: 141-1 and 141-74, 16: control(141-1 and 141-74),
Digestion following plasmid.
| pSB1C3-Km-Blunt |
P1 Preparation following fragment.
| Fragment 1, 2 |
September 23th
September 24th
September 25th
September 26th
September 27th
September 28th
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