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Team:TMU-Tokyo/Notebook experiment/4th
From 2013.igem.org
(Difference between revisions)
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<Br> | <Br> | ||
<b>September 23th</b><Br> | <b>September 23th</b><Br> | ||
| + | PCR following fragment. | ||
| + | <div style="text-align:center;"> | ||
| + | <Table cellpadding="5"> | ||
| + | <Tr> | ||
| + | <Td>Fragment</Td><Td>Fragment 3 - ApCm</Td> | ||
| + | </Tr> | ||
| + | <Tr> | ||
| + | <Td>Primer</Td><Td>mhpA-CmC6<Br>SaApL</Td> | ||
| + | </Tr> | ||
| + | <Tr> | ||
| + | <Td>Templete</Td><Td>Fragment 3 - Cm<Br>Ap(SaApR-SaApL)</Td> | ||
| + | </Tr> | ||
| + | </Table> | ||
| + | </div> | ||
| + | Result.<Br> | ||
| + | <IMG Src="https://static.igem.org/mediawiki/2013/c/cf/TMU-Tokyo_0923.jpg"><Br> | ||
| + | 1: Fragment 3 - Cm, 2: Fragment 3 - ApCm,<Br> | ||
| + | 3: pSB1C3-Km-Blunt<Br> | ||
<Br> | <Br> | ||
<Br> | <Br> | ||
| + | Check PCR.<Br> | ||
| + | <div style="text-align:center;"> | ||
| + | <Table cellpadding="5"> | ||
| + | <Tr> | ||
| + | <Td>Fragment</Td><Td>Fragment 3 - Ap<Br>(control)</Td><Td>Fragment 3 - ApCm</Td> | ||
| + | </Tr> | ||
| + | <Tr> | ||
| + | <Td>Primer</Td><Td>CmNR-Not<Br>SaApL</Td><Td>CmNR-Not<Br>SaApL</Td> | ||
| + | </Tr> | ||
| + | <Tr> | ||
| + | <Td>Templete</Td><Td>(Cell suspention)</Td><Td>(Cell suspention)</Td> | ||
| + | </Tr> | ||
| + | </Table> | ||
| + | </div> | ||
| + | Result.<Br> | ||
| + | <IMG Src="https://static.igem.org/mediawiki/2013/7/7a/TMU-Tokyo_0923_1.jpg"> | ||
| + | <IMG Src="https://static.igem.org/mediawiki/2013/9/94/TMU-Tokyo_0923_2.jpg"><Br> | ||
| + | 1-31: Fragment 3 - ApCm<Br> | ||
| + | <Br> | ||
| + | <Br> | ||
| + | Electro Pration<Br> | ||
| + | <Table><Tr><Td>Fragment 3 - Cm to Escherichia coli MG1655 red.</Td></Tr></Table> | ||
| + | <Br> | ||
| + | <Br> | ||
| + | P1 Preparation<Br> | ||
| + | <Table><Tr><Td>Fragment 3 - ApCm</Td></Tr></Table> | ||
| + | <Br> | ||
| + | <Br> | ||
| + | |||
| + | |||
| + | |||
| + | |||
<b>September 24th</b><Br> | <b>September 24th</b><Br> | ||
| + | P1 transdunction<Br> | ||
| + | <Table><Tr><Td>Fragment 1, 2</Td></Tr></Table> | ||
| + | Result.<Br> | ||
| + | <IMG Src="https://static.igem.org/mediawiki/2013/1/19/TMU-Tokyo_0924.jpg"><Br> | ||
| + | <Br> | ||
| + | <Br> | ||
| + | |||
<b>September 25th</b><Br> | <b>September 25th</b><Br> | ||
Revision as of 22:51, 27 September 2013
Experiment > 4th week
September 22th
Mini prep following plasmid.
| fimE-pBAD(in pSB1A3), pSB1C3-Km, pSB1A3-Km |
1: pSB1C3-Km, 2-4: pSB1A3-Km, 5-6: fimE-pBAD
Check PCR.
| Fragment | (control) | Fragment 3 - ApCm |
| Primer | 141-49 SaApL | 141-49 SaApL |
| Templete | Escherichia coli MG1655 red (Cell suspention) | (Cell suspention) |
1-9: Fragment 3 - ApCm
Check PCR.
| Fragment | (control) | Fragment 3 - ApCm | Fragment 3 - ApCm |
| Primer | 2 sets of the others | 141-1 141-20 | 141-1 141-74 |
| Templete | Escherichia coli MG1655 red (Cell suspention) | (Cell suspention) |
1-7: 141-1 and 141-20, 8: control(141-1 and 141-20),
9-15: 141-1 and 141-74, 16: control(141-1 and 141-74),
Digestion following plasmid.
| pSB1C3-Km-Blunt |
P1 Preparation following fragment.
| Fragment 1, 2 |
September 23th
PCR following fragment.
| Fragment | Fragment 3 - ApCm |
| Primer | mhpA-CmC6 SaApL |
| Templete | Fragment 3 - Cm Ap(SaApR-SaApL) |
1: Fragment 3 - Cm, 2: Fragment 3 - ApCm,
3: pSB1C3-Km-Blunt
Check PCR.
| Fragment | Fragment 3 - Ap (control) | Fragment 3 - ApCm |
| Primer | CmNR-Not SaApL | CmNR-Not SaApL |
| Templete | (Cell suspention) | (Cell suspention) |
1-31: Fragment 3 - ApCm
Electro Pration
| Fragment 3 - Cm to Escherichia coli MG1655 red. |
P1 Preparation
| Fragment 3 - ApCm |
September 24th
P1 transdunction
| Fragment 1, 2 |
September 25th
September 26th
September 27th
September 28th
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