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Team:CU-Boulder/Project/Kit/DNAPurification
From 2013.igem.org
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| - | Columns were used to purify RFP (red) expressing plasmid. Columns were recycled as per the recycling protocol (left) or not (right) and then used to purify AmilCP (blue) expressing plasmid. The elutant was transformed and plated. Recycled columns resulted in 100% AmilCP (blue) producing colonies. Non-recycled columns produced ~ 50% AmilCP (blue) producing colonies and ~50% RFP producing colonies | + | Columns were used to purify RFP (red) expressing plasmid. Columns were recycled as per the recycling protocol (left) or not (right) and then used to purify AmilCP (blue) expressing plasmid. The elutant was transformed and plated. Recycled columns resulted in 100% AmilCP (blue) producing colonies. Non-recycled columns produced ~50% AmilCP (blue) producing colonies and ~50% RFP producing colonies resulting from contaminating plasmid from the previous purification. Recycling works. |
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Revision as of 03:40, 28 September 2013
- Gel Purification of DNA
- This simple procedure utilizes filter paper and dialysis tubing to obtain concentrated DNA from an agarose gel, eliminating the need for commercial gel purification kits.
- Gel Purification of DNA
Video of filter paper+dialysis tubing method of DNA gel purification (500% speed)
- Homemade Miniprep Spin Column and Recycling System
- We developed a method for making our own miniprep spin columns that is much cheaper than commercial plasmid purification kits. Additionally, we tested and validated an effective method of recycling mini-columns to even further reduce cost and waste!
Columns were used to purify RFP (red) expressing plasmid. Columns were recycled as per the recycling protocol (left) or not (right) and then used to purify AmilCP (blue) expressing plasmid. The elutant was transformed and plated. Recycled columns resulted in 100% AmilCP (blue) producing colonies. Non-recycled columns produced ~50% AmilCP (blue) producing colonies and ~50% RFP producing colonies resulting from contaminating plasmid from the previous purification. Recycling works.
