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Team:British Columbia/Notebook/Protocols/Biobrickdigest
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AnnaMüller (Talk | contribs) (Created page with "> {{:Team:British_Columbia/Templates/MainHeader}} ==='''BioBrick Restriction Digest'''=== '''Supplies:''' dsH2O 10x buffer (1,2,3,4) 100x BSA Restriction Enzymes (EcoRI, X...") |
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1. Make a master mix with everything except the DNA. Keep everything on ice. | 1. Make a master mix with everything except the DNA. Keep everything on ice. | ||
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*depending on DNA concentration* | *depending on DNA concentration* | ||
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2. Pipet aliquots to each sample tube and add DNA. | 2. Pipet aliquots to each sample tube and add DNA. | ||
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3. Incubate at 37°C for 2-3h or let digest over night at room temperature. | 3. Incubate at 37°C for 2-3h or let digest over night at room temperature. | ||
Latest revision as of 00:29, 28 September 2013
>
iGEM Home
BioBrick Restriction Digest
Supplies:
dsH2O
10x buffer (1,2,3,4)
100x BSA
Restriction Enzymes (EcoRI, Xbal, SpeI, PstI)
DNA
Steps:
1. Make a master mix with everything except the DNA. Keep everything on ice.
- depending on DNA concentration*
2. Pipet aliquots to each sample tube and add DNA.
3. Incubate at 37°C for 2-3h or let digest over night at room temperature.
