"
Team:Calgary/Notebook/Protocols/PrussianBlueFerritinSynthesis
From 2013.igem.org
(Difference between revisions)
| Line 18: | Line 18: | ||
<li>Add 20µL horsespleen ferritin (47mg/mL) to a solution of 20mL HCl(pH 3.0) and 150µL K<sub>4</sub>Fe(CN)<sub>6</sub> (0.025M).</li> | <li>Add 20µL horsespleen ferritin (47mg/mL) to a solution of 20mL HCl(pH 3.0) and 150µL K<sub>4</sub>Fe(CN)<sub>6</sub> (0.025M).</li> | ||
<li>Dialyze for 12 hours at 4℃ while constantly stirring</li> | <li>Dialyze for 12 hours at 4℃ while constantly stirring</li> | ||
| - | <li>Dialyze 5mL of the previous ferritin solution against sodium acetate-acetic acid buffer (pH 3.6) for 8 hours.</li> | + | <li>Dialyze 5mL of the previous ferritin solution against 500mL of sodium acetate-acetic acid buffer (pH 3.6) for 8 hours.</li> |
<li>Replace buffer and dialyze overnight </li> | <li>Replace buffer and dialyze overnight </li> | ||
</section> | </section> | ||
</html> | </html> | ||
Revision as of 00:52, 28 September 2013
Synthesis
Prussian Blue Ferritin Synthesis
Reagents and Materials
- Horse Spleen Ferritin (47mg/mL)
- Hydrogen Chloride (pH 3.0)
- K4Fe(CN)6 (0.025M)
- Sodium acetate-acetic acid buffer (pH 3.6)
- Add 20µL horsespleen ferritin (47mg/mL) to a solution of 20mL HCl(pH 3.0) and 150µL K4Fe(CN)6 (0.025M).
- Dialyze for 12 hours at 4℃ while constantly stirring
- Dialyze 5mL of the previous ferritin solution against 500mL of sodium acetate-acetic acid buffer (pH 3.6) for 8 hours.
- Replace buffer and dialyze overnight
