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Team:Paris Saclay/Notebook/August/23
From 2013.igem.org
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===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3'''==== | ===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3'''==== | ||
| - | ===='''1 - Electrophoresis to check the digestion of Bba_J04450 by EcoRI/PstI'''==== | + | ===='''1 - Digestion of Bba_J04450 by EcoRI/PstI'''==== |
| + | |||
| + | XioaJing | ||
| + | |||
| + | Used quantities : | ||
| + | * Buffer FD: 2µL | ||
| + | * H2O : 5µL | ||
| + | * DNA : 9µL | ||
| + | * EcoRI FD : 1µL | ||
| + | * PstI FD : 1µL | ||
| + | |||
| + | We let the digestion at 37°C during 10 minutes. | ||
| + | |||
| + | ===='''2 - Electrophoresis to check the digestion of Bba_J04450 by EcoRI/PstI'''==== | ||
{| | {| | ||
Revision as of 18:26, 28 September 2013
Contents |
Notebook : August 23
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3
1 - Digestion of Bba_J04450 by EcoRI/PstI
XioaJing
Used quantities :
- Buffer FD: 2µL
- H2O : 5µL
- DNA : 9µL
- EcoRI FD : 1µL
- PstI FD : 1µL
We let the digestion at 37°C during 10 minutes.
2 - Electrophoresis to check the digestion of Bba_J04450 by EcoRI/PstI
| ]] |
|
Expect sizes :
- PSB3K3 : 2750 bp
|
We can see anything. The digestion did work. |
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