Team:Paris Saclay/Notebook/August/7

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Protocol : [[Team:Paris_Saclay/Protocols/transduction|Transduction]]
Protocol : [[Team:Paris_Saclay/Protocols/transduction|Transduction]]
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Our Mutant bacteria was called BW : Δfnr::Km.
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Our wild type bacteria was called MG1655Z1.
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We did the first step of the protocol : bacteriophage stock which packed Km gene. 
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Revision as of 14:25, 28 September 2013

Contents

Notebook : August 7

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155007

1 - Tranformation Bba_K115507 in DH5α

XiaoJing

Protocol : Bacterial transformation

Objective : characterize Bba_K1155000, Bba_K1155004, Bba_K1155005, Bba_K1155006

1 - Tranduction of Km in MG1655Z1

Nadia, XioaJing

Protocol : Transduction

Our Mutant bacteria was called BW : Δfnr::Km. Our wild type bacteria was called MG1655Z1.

We did the first step of the protocol : bacteriophage stock which packed Km gene.

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