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Team:Paris Saclay/Notebook/August/7
From 2013.igem.org
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===='''Objective : obtaining Bba_K1155007'''==== | ===='''Objective : obtaining Bba_K1155007'''==== | ||
| - | ===='''1 - Tranformation Bba_K115507 in DH5α==== | + | ===='''1 - Ligation of RBS-LacZ-Term in PSB1C3 ==== |
| + | |||
| + | Anaïs, Nadia | ||
| + | |||
| + | Used quantities : | ||
| + | * RBS-AmilCP : 2µL | ||
| + | * Term in PSB1C3 : 2.5µL | ||
| + | * Buffer : 1µL | ||
| + | * Ligase : 1µL | ||
| + | * H2O : 3.5µL | ||
| + | |||
| + | ===='''2 - Tranformation Bba_K115507 in DH5α==== | ||
XiaoJing | XiaoJing | ||
Revision as of 21:38, 28 September 2013
Contents |
Notebook : August 7
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155007
1 - Ligation of RBS-LacZ-Term in PSB1C3
Anaïs, Nadia
Used quantities :
- RBS-AmilCP : 2µL
- Term in PSB1C3 : 2.5µL
- Buffer : 1µL
- Ligase : 1µL
- H2O : 3.5µL
2 - Tranformation Bba_K115507 in DH5α
XiaoJing
Protocol : Bacterial transformation
Objective : characterize Bba_K1155000, Bba_K1155004, Bba_K1155005, Bba_K1155006
1 - Tranduction of Km in MG1655Z1
Nadia, XioaJing
Protocol : Transduction
Our Mutant bacteria was called BW : Δfnr::Km. Our wild type bacteria was called MG1655Z1.
We did the first step of the protocol : bacteriophage stock which packed Km gene.
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