Collaboration

All of the work that was done during this project was both planned and executed by our iGEM team members. It all started of with some brainstorming sessions that began in the middle of May, although the actual lab work began in the beginning of June.

During the summer we have worked very closely with the Linköpings iGEM team. Since it was their first year to participate in the competition we decided to do our best to provide them with any assistance they needed, such as providing them with parts, knowledge etc. In exchange, they provided us with some very valuable information about basic modeling that we could use when modeling our own system.

In addition to assisting Linköpings iGEM team we have also sent biobricks to the following iGEM teams:


UT-Tokyo iGEM: BBa_K592010 pSB1C3-amilGFP BBa_K864401 pSB1C3-aeBlue


iGEM Braunschweig: BBa_K592010 pSB1C3-amilGFP BBa_K592011 pSB1C3-cjBlue BBa_K592012 pSB1C3-eforRed BBa_K592025 pSB1C3-B0034-amilCP BBa_K864401 pSB1C3-aeBlue


Tsinghua iGEM 2013: BBa_K592010 pSB1C3-amilGFP BBa_K592011 pSB1C3-cjBlue BBa_K592012 pSB1C3-eforRed BBa_K592025 pSB1C3-B0034-amilCP BBa_K864401 pSB1C3-aeBlue


CU Boulder iGEM: BBa_K592010 pSB1C3-amilGFP BBa_K592011 pSB1C3-cjBlue BBa_K592012 pSB1C3-eforRed BBa_K592025 pSB1C3-B0034-amilCP BBa_K864401 pSB1C3-aeBlue


uOttawa yellow chromoprotein: BBa_K592010 red chromoproteins: BBa_K592012


HokkaidoU K592009 – AmilCP (with RBS) K592010 – AmilGFP (with and without RBS) K592011 – CjBlue (only without RBS) K592012 – eforRed (only without RBS) K864401 – aeBlue (only without RBS)


TU Darmstadt pSB1C3-eforRed pSB1C3-CjBlue pSB1C3-B0034-aeBlue pSB1K3-J23110-B0034-aeBlue pSB1C3-B0034-amilGFP pSB1C3-B0034-amilCP pSB1C3-J23110-B0034-tsPurple


We would also like to thank other sources that have helped us, such as the Slovenian iGEM team 2010 that suppied us with the genes needed for the production of carotenoid metabolites, TU-Munichs iGEM team 2012 that provided us with the gene 4CL for the production of 4-coumaryl-CoA, last but not least we would also like to thank the School of chemical and Biomolecular Engineering, Cornell University for providing us with the genes involved in the production of the phenylpropanoid pathway. The article can be found at; Conrado RJ, Mori J et al (2011)


In addition to this we would also like to mention some fascinating requests that have been made by external sources:

Nature reviews request to display our chromoproteins on their front page for their 10 year anniversary

National Aeronautics and Space Administrations (NASA) biotechnology department requested our chromoproteins as reporter genes for a project that is currently being worked on.