Edinburgh University

From 2013.igem.org

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We have decided to work with ''Bacillus subtilis'' as our chassis. Using it we want to solve the problem of organic and inorganic environmental pollution coming from food and beverage industry.
 
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Our first goal will be to detect heavy metal ions. This would be followed by metal ion binding using peptides synthesised by both ribosome and a non-ribosomal peptide synthase (NRPS). To isolate the ions from the waste we will try to control biofilm formation and lock toxic metals bound to peptides in it.
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The Edinburgh iGEM 2013 team, WastED, is focusing on remediation and valorization of industrial waste streams, with a particular focus on Scottish leather and whisky industry waste waters, containing toxic heavy metal ions as well as fermentable organic components. Using <i>Bacillus subtilis</i> as chassis, we are engineering organisms to capture ions using chelators and metal binding proteins, and to ferment organic components to produce biofuels. We are also testing a new assembly procedure, GenBrick, based on the Genabler assembly system. GenBrick allows assembly of multiple RFC10-compatible BioBricks in a single reaction, and is also well suited to the preparation of fusion proteins and addition of terminal tags. Enzyme fusions may enhance metabolic pathways through substrate channeling. We are testing the effect of protein fusions on fermentation efficiency for biofuel production. In addition, we are examining the implications of possible Scottish independence, following the 2014 referendum, for Synthetic Biology in Scotland.
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To utilise the organic components of industry waste we want to produce bioethanol from it.  
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We hope that the project will increase the amount of useful parts available for ''B. subtilis'' (mainly the metal activated promoters and Non-ribosomal peptide synthase domains). Creation of large multi-domain enzymes and fusion proteins which is required for our project will be achieved using ground-breaking GenBrick technology which will be introduced to the iGEM community for the first time.
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Revision as of 17:01, 3 October 2013

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WastED

The Edinburgh iGEM 2013 team, WastED, is focusing on remediation and valorization of industrial waste streams, with a particular focus on Scottish leather and whisky industry waste waters, containing toxic heavy metal ions as well as fermentable organic components. Using Bacillus subtilis as chassis, we are engineering organisms to capture ions using chelators and metal binding proteins, and to ferment organic components to produce biofuels. We are also testing a new assembly procedure, GenBrick, based on the Genabler assembly system. GenBrick allows assembly of multiple RFC10-compatible BioBricks in a single reaction, and is also well suited to the preparation of fusion proteins and addition of terminal tags. Enzyme fusions may enhance metabolic pathways through substrate channeling. We are testing the effect of protein fusions on fermentation efficiency for biofuel production. In addition, we are examining the implications of possible Scottish independence, following the 2014 referendum, for Synthetic Biology in Scotland.


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This iGEM team has been funded by the MSD Scottish Life Sciences Fund. The opinions expressed by this iGEM team are those of the team members and do not necessarily represent those of MSD
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