Team:TU-Delft/Notebook/2013/09/29/

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Revision as of 19:21, 3 October 2013

Notebook

29th of September

Lab work

1. Membrane experiment is carried out to quantify the AIPs diffusion across the membrane for the band-aid model.
2. Sequence coverage from Tandem MS for Signiferin Peptide :

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-== 28th of September==
+== 29th of September==
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 . Membrane experiment is carried out to quantify the AIPs diffusion across the membrane for the band-aid model.<br>
-. The corrected construct of the pBAD AIP Receiver GFP colonies 6, 7 and 8 were induced with Arabinose 0.1% and AIP 1 µM and 10 µM. Controls of No Arabinose and No AIP, No Arabinose and only AIP, No AIP and only arabinose induction were also done. <br>
+. Sequence coverage from Tandem MS for Signiferin Peptide : <br>
+<center>
-Hours after AIP induction the samples were analyzed on FACS for signals of GFP. <br>
+<img src="https://static.igem.org/mediawiki/2013/0/0c/Sumo4.png">
+</center>
-. pBAD Ulp TT pT7 SUMO Signiferin construct was induced with Arabinose and IPTG to check for the cleavage of SUMO-Peptide by Ulp protease. The cells were french pressed and stored in -80 degree. MIC experiment will be carried out on it and an SDS Page will be run to check for Ulp, SUMO-Peptide and cleaved peptide bands.<br>
-. Samples of the pT7 Sumo peptides were given for MS measurements. <br>

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