Team:Paris Saclay/Primers

From 2013.igem.org

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| pSB1C3, E.coli
| pSB1C3, E.coli
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|-
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! scope="row"|  
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! scope="row"| iPS54
-
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| Bba_BphR2-part1-Gibson-Forward
-
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| GAATTCGCGGCCGCTTCTAGAGATGGAACTGCATGACCTGGAT
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|  
+
| P. pseuoalcaligenes
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|-
-
! scope="row"|  
+
! scope="row"| iPS55
-
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+
| Bba_BphR2-part1-Gibson-Reverse
-
|  
+
| CAGTTCGTCCTGTAGTGCGG
-
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+
| P. pseuoalcaligenes
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|-
-
! scope="row"|  
+
! scope="row"| iPS56
-
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| Bba_BphR2-part2-Gibson-Forward
-
|  
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| CACCGCACTACAGGACGAAC
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+
| P. pseuoalcaligenes
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|-
-
! scope="row"|  
+
! scope="row"| iPS57
-
|  
+
| Bba_BphR2-part2-Gibson-Reverse
-
|  
+
| CTGCAGCGGCCGCTACTAGTATCAATCCGAAAACAGCTCGAACA
-
|  
+
| P. pseuoalcaligenes
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|-
-
! scope="row"|  
+
! scope="row"| iPS58
-
|  
+
| Bba_RBS-BphR2-part1-Gibson-Forward
-
|  
+
| GAATTCGCGGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGAACTGCATGACCTGGAT
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|  
+
| P. pseuoalcaligenes
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|-
-
! scope="row"|  
+
! scope="row"| iPS59
-
|  
+
| Bba_FNR-part1-Gibson-Forward
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|  
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| GAATTCGCGGCCGCTTCTAGAGATGATCCCGGAAAAGCGAAT
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|  
+
| E.coli
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|-
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! scope="row"|  
+
! scope="row"| iPS60
-
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| Bba_FNR-part1-Gibson-Reverse
-
|  
+
| CGGAACTCACGAGGGGAGAA
-
|  
+
| E.coli
 +
|-
 +
! scope="row"| iPS61
 +
| Bba_FNR-part2-Gibson-Forward
 +
| TTCTCCCCTCGTGAGTTCCG
 +
| E.coli
 +
|-
 +
! scope="row"| iPS62
 +
| Bba_FNR-part2-Gibson-Reverse
 +
| CTGCAGCGGCCGCTACTAGTATCAGGCAACGTTACGCGTAT
 +
| E.coli
 +
|-
 +
! scope="row"| iPS63
 +
| Bba_RBS-FNR-part1-Gibson-Forward
 +
| GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGATCCCGGAAAAGCGAATT
 +
| E.coli
 +
|-
 +
! scope="row"| iPS64
 +
| Suffix-from-pSB1C3-Gibson
 +
| TACTAGTAGCGGCCGCTGCAG
 +
| pSB1C3, E.coli
 +
|-
 +
! scope="row"| iPS65
 +
| Prefix-from-pSB1C3-Gibson
 +
| CTCTAGAAGCGGCCGCGAATTC
 +
| pSB1C3, E.coli
|-
|-

Revision as of 21:25, 3 October 2013

Labwork Overview Biobricks Protocols Primers Safety

Number Name Sequence Organism
iPS33 pfnr_up ATATGAATTCGCGGCCGCTTCTAGAGT E.coli
iPS34 pfnr_down ATATCTGCAGCGGCCGCTACTAGTAGTTCTTCGCTATTGAGAATAACATG E.coli
iPS35 bphR2_up ATATGAATTCGCGGCCGCTTCTAGATGGAACTGCATGACCTGGA P. pseuoalcaligenes
iPS36 bphR2_down ATATCTGCAGCGGCCGCTACTAGTATCAATCCGAAAACAGCTCGA P. pseuoalcaligenes
iPS37 bphR1_up ATATGAATTCGCGGCCGCTTCTAGAG ATGTCCCTGGGTGACATGCGTG P. pseuoalcaligenes
iPS38 bphR1_down ATATCTGCAGCGGCCGCTACTAGTA AGCAGTCAATCGGCGGTAGCGC P. pseuoalcaligenes
iPS39 bphA1_up ATATGAATTCGCGGCCGCTTCTAGAG CGCCAACATCCCACGTGTTTG P. pseuoalcaligenes
iPS40 bphA1_down ATATCTGCAGCGGCCGCTACTAGTACCCCCGGATCGCCTCCGGCGTC P. pseuoalcaligenes
iPS41 SB-prep-3P-1 GCCGCTGCAGTCCGGCAAAAAA pSB1C3, E.coli
iPS42 SB-prep-2Ea TGCCACCTGACGTCTAAGAA pSB1C3, E.coli
iPS43 VF2 ATTACCGCCTTTGAGTGAGC pSB1C3, E.coli
iPS44 VR ATTACCGCCTTTGAGTGAGC pSB1C3, E.coli
iPS45 Prefix-prom _nirB TCATGAATTCGCGGCCGCTTCTAGAGCTACGTGCATCTATTTCTAT E.coli
iPS46 suffixe-prom_nirB TACTCTGCAGCGGCCGCTACTAGTATGCCTCGATTTCTTTTCTAT E.coli
iPS47 Prefix-prom _narK TCATGAATTCGCGGCCGCTTCTAGAGACATCGGTAAGGGTAGGGAT E.coli
iPS48 suffixe-prom_narK TACTCTGCAGCGGCCGCTACTAGTAAGACACCTCTGATACTCGTT E.coli
iPS49 prefix-prom _narG TCATGAATTCGCGGCCGCTTCTAGAGGGAATTTACTTTATTTTTCA E.coli
iPS50 suffixe-prom_narG TACTCTGCAGCGGCCGCTACTAGTATTCTCCTGTGGGAGCCTGTC E.coli
iPS51 Prefix from pSB1C3 CTCTAGAAGCGGCCGCGAATTC pSB1C3, E.coli
iPS52 sufix from pSB1C3 GTACCTGCAGCGGCCGCTACTAGTA pSB1C3, E.coli
iPS53 prefix -RBS-AmilCP CATAGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGTGTGATCGCTAAACAAAT pSB1C3, E.coli
iPS54 Bba_BphR2-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGATGGAACTGCATGACCTGGAT P. pseuoalcaligenes
iPS55 Bba_BphR2-part1-Gibson-Reverse CAGTTCGTCCTGTAGTGCGG P. pseuoalcaligenes
iPS56 Bba_BphR2-part2-Gibson-Forward CACCGCACTACAGGACGAAC P. pseuoalcaligenes
iPS57 Bba_BphR2-part2-Gibson-Reverse CTGCAGCGGCCGCTACTAGTATCAATCCGAAAACAGCTCGAACA P. pseuoalcaligenes
iPS58 Bba_RBS-BphR2-part1-Gibson-Forward GAATTCGCGGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGAACTGCATGACCTGGAT P. pseuoalcaligenes
iPS59 Bba_FNR-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGATGATCCCGGAAAAGCGAAT E.coli
iPS60 Bba_FNR-part1-Gibson-Reverse CGGAACTCACGAGGGGAGAA E.coli
iPS61 Bba_FNR-part2-Gibson-Forward TTCTCCCCTCGTGAGTTCCG E.coli
iPS62 Bba_FNR-part2-Gibson-Reverse CTGCAGCGGCCGCTACTAGTATCAGGCAACGTTACGCGTAT E.coli
iPS63 Bba_RBS-FNR-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGATCCCGGAAAAGCGAATT E.coli
iPS64 Suffix-from-pSB1C3-Gibson TACTAGTAGCGGCCGCTGCAG pSB1C3, E.coli
iPS65 Prefix-from-pSB1C3-Gibson CTCTAGAAGCGGCCGCGAATTC pSB1C3, E.coli

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