Team:Paris Saclay/Primers

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(Difference between revisions)

Revision as of 21:46, 3 October 2013

Labwork Overview Biobricks Protocols Primers Safety

Number	Name	Sequence	Organism
iPS33	pfnr_up	ATATGAATTCGCGGCCGCTTCTAGAGT	E.coli
iPS34	pfnr_down	ATATCTGCAGCGGCCGCTACTAG TAGTTCTTCGCTATTGAGAATAACATG	E.coli
iPS35	bphR2_up	ATATGAATTCGCGGCCGCTTCTAGA TGGAACTGCATGACCTGGA	P. pseuoalcaligenes
iPS36	bphR2_down	ATATCTGCAGCGGCCGCTACTAGTAT CAATCCGAAAACAGCTCGA	P. pseuoalcaligenes
iPS37	bphR1_up	ATATGAATTCGCGGCCGCTTCTAGAG ATGTCCCTGGGTGACATGCGTG	P. pseuoalcaligenes
iPS38	bphR1_down	ATATCTGCAGCGGCCGCTACTAGTAA GCAGTCAATCGGCGGTAGCGC	P. pseuoalcaligenes
iPS39	bphA1_up	ATATGAATTCGCGGCCGCTTCTAGAGC GCCAACATCCCACGTGTTTG	P. pseuoalcaligenes
iPS40	bphA1_down	ATATCTGCAGCGGCCGCTACTAGTACC CCCGGATCGCCTCCGGCGTC	P. pseuoalcaligenes
iPS41	SB-prep-3P-1	GCCGCTGCAGTCCGGCAAAAAA	pSB1C3, E.coli
iPS42	SB-prep-2Ea	TGCCACCTGACGTCTAAGAA	pSB1C3, E.coli
iPS43	VF2	ATTACCGCCTTTGAGTGAGC	pSB1C3, E.coli
iPS44	VR	ATTACCGCCTTTGAGTGAGC	pSB1C3, E.coli
iPS45	Prefix-prom _nirB	TCATGAATTCGCGGCCGCTTCTAGA GCTACGTGCATCTATTTCTAT	E.coli
iPS46	suffixe-prom_nirB	TACTCTGCAGCGGCCGCTACTAGTA TGCCTCGATTTCTTTTCTAT	E.coli
iPS47	Prefix-prom _narK	TCATGAATTCGCGGCCGCTTCTAGAG ACATCGGTAAGGGTAGGGAT	E.coli
iPS48	suffixe-prom_narK	TACTCTGCAGCGGCCGCTACTAGTAA GACACCTCTGATACTCGTT	E.coli
iPS49	prefix-prom _narG	TCATGAATTCGCGGCCGCTTCTAGAG GGAATTTACTTTATTTTTCA	E.coli
iPS50	suffixe-prom_narG	TACTCTGCAGCGGCCGCTACTAGTAT TCTCCTGTGGGAGCCTGTC	E.coli
iPS51	Prefix from pSB1C3	CTCTAGAAGCGGCCGCGAATTC	pSB1C3, E.coli
iPS52	sufix from pSB1C3	GTACCTGCAGCGGCCGCTACTAGTA	pSB1C3,E.coli
iPS53	prefix -RBS-AmilCP	CATAGAATTCGCGGCCGCTTCTAGAGAAAG AGGAGAAATACTAGATGAGTGTGATCGCTAAACAAAT	pSB1C3,E.coli
iPS54	Bba_BphR2-part1-Gibson-Forward	GAATTCGCGGCCGCTTCTAGAGATGGAA CTGCATGACCTGGAT	P. pseuoalcaligenes
iPS55	Bba_BphR2-part1-Gibson-Reverse	CAGTTCGTCCTGTAGTGCGG	P. pseuoalcaligenes
iPS56	Bba_BphR2-part2-Gibson-Forward	CACCGCACTACAGGACGAAC	P. pseuoalcaligenes
iPS57	Bba_BphR2-part2-Gibson-Reverse	CTGCAGCGGCCGCTACTAGTATCAATCCG AAAACAGCTCGAACA	P. pseuoalcaligenes
iPS58	Bba_RBS-BphR2-part1-Gibson-Forward	GAATTCGCGGGCCGCTTCTAGAGAAAGAGG AGAAATACTAGATGGAACTGCATGACCTGGAT	P. pseuoalcaligenes
iPS59	Bba_FNR-part1-Gibson-Forward	GAATTCGCGGCCGCTTCTAGAGATGATCCC GGAAAAGCGAAT	E.coli
iPS60	Bba_FNR-part1-Gibson-Reverse	CGGAACTCACGAGGGGAGAA	E.coli
iPS61	Bba_FNR-part2-Gibson-Forward	TTCTCCCCTCGTGAGTTCCG	E.coli
iPS62	Bba_FNR-part2-Gibson-Reverse	CTGCAGCGGCCGCTACTAGTATCAGGCAAC GTTACGCGTAT	E.coli
iPS63	Bba_RBS-FNR-part1-Gibson-Forward	GAATTCGCGGCCGCTTCTAGAGAAAGAGGA GAAATACTAGATGATCCCGGAAAAGCGAATT	E.coli
iPS64	Suffix-from-pSB1C3-Gibson	TACTAGTAGCGGCCGCTGCAG	pSB1C3,E.coli
iPS65	Prefix-from-pSB1C3-Gibson	CTCTAGAAGCGGCCGCGAATTC	pSB1C3,E.coli

@@ Line 32: / Line 32: @@
 ! scope="row"| iPS35
 | bphR2_up
-| ATATGAATTCGCGGCCGCTTCTAGATGGAACTGCATGACCTGGA
+| ATATGAATTCGCGGCCGCTTCTAGA
+TGGAACTGCATGACCTGGA
 | P. pseuoalcaligenes
 |-
 ! scope="row"| iPS36
 | bphR2_down
-| ATATCTGCAGCGGCCGCTACTAGTATCAATCCGAAAACAGCTCGA
+| ATATCTGCAGCGGCCGCTACTAGTAT
+CAATCCGAAAACAGCTCGA
 | P. pseuoalcaligenes
 |-
 ! scope="row"| iPS37
 | bphR1_up
-| ATATGAATTCGCGGCCGCTTCTAGAGATGTCCCTGGGTGACATGCGTG
+| ATATGAATTCGCGGCCGCTTCTAGAG
+ATGTCCCTGGGTGACATGCGTG
 | P. pseuoalcaligenes
 |-
 ! scope="row"| iPS38
 | bphR1_down
-| ATATCTGCAGCGGCCGCTACTAGTAAGCAGTCAATCGGCGGTAGCGC
+| ATATCTGCAGCGGCCGCTACTAGTAA
+GCAGTCAATCGGCGGTAGCGC
 | P. pseuoalcaligenes
 |-
 ! scope="row"| iPS39
 | bphA1_up
-| ATATGAATTCGCGGCCGCTTCTAGAGCGCCAACATCCCACGTGTTTG
+| ATATGAATTCGCGGCCGCTTCTAGAGC
+GCCAACATCCCACGTGTTTG
 | P. pseuoalcaligenes
 |-
 ! scope="row"| iPS40
 | bphA1_down
-| ATATCTGCAGCGGCCGCTACTAGTACCCCCGGATCGCCTCCGGCGTC
+| ATATCTGCAGCGGCCGCTACTAGTACC
+CCCGGATCGCCTCCGGCGTC
 | P. pseuoalcaligenes
 |-
@@ Line 82: / Line 88: @@
 ! scope="row"| iPS45
 | Prefix-prom _nirB
-| TCATGAATTCGCGGCCGCTTCTAGAGCTACGTGCATCTATTTCTAT
+| TCATGAATTCGCGGCCGCTTCTAGA
+GCTACGTGCATCTATTTCTAT
 | E.coli
 |-
 ! scope="row"| iPS46
 | suffixe-prom_nirB
-| TACTCTGCAGCGGCCGCTACTAGTATGCCTCGATTTCTTTTCTAT
+| TACTCTGCAGCGGCCGCTACTAGTA
+TGCCTCGATTTCTTTTCTAT
 | E.coli
 |-
 ! scope="row"| iPS47
 | Prefix-prom _narK
-| TCATGAATTCGCGGCCGCTTCTAGAGACATCGGTAAGGGTAGGGAT
+| TCATGAATTCGCGGCCGCTTCTAGAG
+ACATCGGTAAGGGTAGGGAT
 | E.coli
 |-
 ! scope="row"| iPS48
 | suffixe-prom_narK
-| TACTCTGCAGCGGCCGCTACTAGTAAGACACCTCTGATACTCGTT
+| TACTCTGCAGCGGCCGCTACTAGTAA
+GACACCTCTGATACTCGTT
 | E.coli
 |-
 ! scope="row"| iPS49
 | prefix-prom _narG
-| TCATGAATTCGCGGCCGCTTCTAGAGGGAATTTACTTTATTTTTCA
+| TCATGAATTCGCGGCCGCTTCTAGAG
+GGAATTTACTTTATTTTTCA
 | E.coli
 |-
 ! scope="row"| iPS50
 | suffixe-prom_narG
-| TACTCTGCAGCGGCCGCTACTAGTATTCTCCTGTGGGAGCCTGTC
+| TACTCTGCAGCGGCCGCTACTAGTAT
+TCTCCTGTGGGAGCCTGTC
 | E.coli
 |-
@@ Line 122: / Line 134: @@
 ! scope="row"| iPS53
 | prefix -RBS-AmilCP
-| CATAGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGTGTGATCGCTAAACAAAT
+| CATAGAATTCGCGGCCGCTTCTAGAGAAAG
+AGGAGAAATACTAGATGAGTGTGATCGCTAAACAAAT
 | pSB1C3,E.coli
 |-
 ! scope="row"| iPS54
 | Bba_BphR2-part1-Gibson-Forward
-| GAATTCGCGGCCGCTTCTAGAGATGGAACTGCATGACCTGGAT
+| GAATTCGCGGCCGCTTCTAGAGATGGAA
+CTGCATGACCTGGAT
 | P. pseuoalcaligenes
 |-
@@ Line 142: / Line 156: @@
 ! scope="row"| iPS57
 | Bba_BphR2-part2-Gibson-Reverse
-| CTGCAGCGGCCGCTACTAGTATCAATCCGAAAACAGCTCGAACA
+| CTGCAGCGGCCGCTACTAGTATCAATCCG
+AAAACAGCTCGAACA
 | P. pseuoalcaligenes
 |-
 ! scope="row"| iPS58
 | Bba_RBS-BphR2-part1-Gibson-Forward
-| GAATTCGCGGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGAACTGCATGACCTGGAT
+| GAATTCGCGGGCCGCTTCTAGAGAAAGAGG
+AGAAATACTAGATGGAACTGCATGACCTGGAT
 | P. pseuoalcaligenes
 |-
 ! scope="row"| iPS59
 | Bba_FNR-part1-Gibson-Forward
-| GAATTCGCGGCCGCTTCTAGAGATGATCCCGGAAAAGCGAAT
+| GAATTCGCGGCCGCTTCTAGAGATGATCCC
+GGAAAAGCGAAT
 | E.coli
 |-
@@ Line 167: / Line 184: @@
 ! scope="row"| iPS62
 | Bba_FNR-part2-Gibson-Reverse
-| CTGCAGCGGCCGCTACTAGTATCAGGCAACGTTACGCGTAT
+| CTGCAGCGGCCGCTACTAGTATCAGGCAAC
+GTTACGCGTAT
 | E.coli
 |-
 ! scope="row"| iPS63
 | Bba_RBS-FNR-part1-Gibson-Forward
-| GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGATCCCGGAAAAGCGAATT
+| GAATTCGCGGCCGCTTCTAGAGAAAGAGGA
+GAAATACTAGATGATCCCGGAAAAGCGAATT
 | E.coli
 |-