May

Milestones

• Initiating lab work on the sub-project endogenous mediator Glycerol dehydrogenase.
• Starting lab work with the first successful PCR: GldA with Pre- and Suffix overlaps could be amplified from Escherichia coli genome.
• The main work however is still planning of our subprojects, designing experiments and a lot of research.
• Finding sponsors goes ahead. Many companies like our project and want to support us.

Week 1

Organization

• We were already able to find sponsors for our team: IIT BIEKUBA, IIT Biotech, Evonik, PlasmidFactory and FisherScientific will support us.

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Mediators

• Glycerol dehydrogenase
  • Primerdesign for isolation of GldA gene from Escherichia coli KRX strain, with overlaps for BioBrick Prefix and Suffix:
  • Forward Primer GldA (43 bp): GAATTCGCGGCCGCTTCTAGATGGACCGCATTATTCAATCACC
  • Reverse Primer GldA (45 bp): CTGCAGCGGCCGCTACTAGTATTATTCCCACTCTTGCAGGAAACG

2.Week

Organization

• Distribution of our team in various subgroups for best work efficiency.
• We’ve organized a barbecue to thank the working group of Dr. Jörn Kalinowski in the CeBiTec for the appropriation of space and support in the laboratory.

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3.Week

Organization

• Planning the trip to the European jamboree in Lyon from October 11. to 13. 2013.

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Mediators

• Glycerol dehydrogenase
  • Starting first cultivation of Escherichia coli KRX strain for complete genome isolation.
  • Successful genome isolation of Escherichia coli KRX.
  • Successful PCR on the GldA gene of Escherichia coli KRX strain.

Table 1: Standard Phusion PCR Master Mix.

Table 2: Two step standard Phusion PCR program for GldA amplification.

• • GldA PCR product was isolated by agarose gel electrophoresis and purified.
  • Bands are on at the expected size of 1050 bp.

Figure 1: Agarose gel from PCR on the GldA gene of Escherichia coli KRX strain with forward and reverse primer GldA. As a ladder we used [http://www.thermoscientificbio.com/nucleic-acid-electrophoresis/generuler-1-kb-dna-ladder-ready-to-use-250-to-10000-bp GeneRuler™ 1 kb DNA Ladder from Thermo Scientific].

4.Week

Organization

• We will also take part at the congress "Next generation of biotechnological processes 2020+" in Berlin at 27. June 2013.
• Having a second presentation at Merck KGaA head office in Darmstadt for explaining our project in detail. We are happy that Merck will be our next supporter in 2013.

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