Team:Evry/vf

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<h1> Bienvenue sur le projet <b><span style="color:#bb8900">Iron coli</span></b></h1>
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<p align="center"><img src="https://static.igem.org/mediawiki/2013/8/86/6c65e704ce82f8a0c01ce08e5c6fe684.png" alt="Warning" width="3%"/><span style="color:#FF0000"><b>Please keep in mind that this project is still under research and there is no clinical testing. Nous sommes un groupe d'étudiants participant à la compétition IGEM. Si vous avez des questions, contactez-nous à : <u>igemevry@gmail.com</u></b></span></p>
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<h1>Welcome to the <b><span style="color:#bb8900">Iron Coli Project</span></b> Home Page</h1>
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<p align="center"><b>The aim of our team this year is to design and realize a <em>treatment for hemochromatosis and thalassemia</em> using synthetic biology with an <em>integrated human practice</em> and <em>modeling approach</em>. </b></p>
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<h3 align="center">Abstract</h3>
<h3 align="center">Abstract</h3>
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Cette année, notre projet porte sur les troubles hématologiques. Plus précisément sur les maladies causant une surcharge sanguine en fer tel que l'hémochromatose et les thalassémies. Pour l'hémochromatose héréditaire, les symptômes sont dus à une surabsorption de fer dans le duodénum, causé par la mutation de la protéine HFE. Cependant, dans le cas des thalassémies, les patients absorbent excessivement le fer à cause de perturbation métabolique.  
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Notre projet on developing a novel treatment for <a href="https://2013.igem.org/Team:Evry/Project_diseases"><em>hematological disorders</em></a>  caused by an <a href="https://2013.igem.org/Team:Evry/Project_metabolism"><em/>iron overload</em></a>, such as hemochromatosis and thalassemia. These autosomal recessive disorders have symptoms including cirrhosis, arthritis, and heart failure, which result from overabsorption of iron from the duodenum. Although these are among the most common heritable diseases, treatment options are limited. Even today patients are mostly treated by frequent bloodletting, which <a href="https://2013.igem.org/Team:Evry/HumanPractice/Patients_perceptions"><em>many people cannot support</em></a>. The aim of our project is to combat these diseases at the source by developing a therapy that prevents the intestinal absorption of iron.
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Actuellement, le fer en surcharge est principalement traité par des saignées pour les patients atteints d'hémochromatose mais ce traitement n'est pas applicable aux thalassémiques qui souffre d'anémie. Le but de notre projet est de limiter l'absorption intestinale du fer en agissant directement à la source.
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We engineer the <i>Escherichia coli</i> <a href="https://2013.igem.org/Team:Evry/Project_FUR"><em>Ferric Uptake Regulation</em></a> (FUR) system using a <a href="https://2013.igem.org/Team:Evry/Inverter"><em>genetic inverter</em></a> so that they produce siderophores (<a href="https://2013.igem.org/Team:Evry/Chelator"><em>iron chelators</em></a>) in response of high concentrations of iron. These engineered bacteria are delivered to the patient's intestine by encapsulating them in an ingestible polymer (<a href="https://2013.igem.org/Team:Evry/Pill_design"><em>capsule</em></a>)  that specifically degrades in the duodenum. Once released into the intestine, the bacteria respond to ambient iron by secreting elevated levels of siderophores, thereby chelating the iron to prevent its absorption by the patient.
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En utilisant le système FUR (Ferric Uptake Regulation) qui contrôle la biosynthèse de sidérophore (des chélateurs de fer), nous modifions <i>Escherichia coli</i> afin qu'elle produise ces sidérophores en réponse à de fortes concentrations en fer. Pour réduire l'absorption du fer chez le patient, notre bactérie sera placée dans un comprimé qui sera ingéré lors d'un repas. Dès qu'elle arrivera dans le duodénum, notre bactérie produira les sidérophores à leur plein potentiel et chélatera le fer.
 
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<h3 align="center">Overview of the project and the achievements</h3>
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alert("Our team is proud to announce that we were awarded a gold medal and the Best Human Practice price at the european jamboree! We are going to Boston.\n\nUnfortunately, we are missing a few thousands of euros to be capable of finishing the competition. If you are a company and would like to sponsor our team, please contact us at igemevry@gmail.com.")
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Revision as of 16:12, 17 October 2013

Iron coli project


WarningPlease keep in mind that this project is still under research and there is no clinical testing. Nous sommes un groupe d'étudiants participant à la compétition IGEM. Si vous avez des questions, contactez-nous à : igemevry@gmail.com


Welcome to the Iron Coli Project Home Page


The aim of our team this year is to design and realize a treatment for hemochromatosis and thalassemia using synthetic biology with an integrated human practice and modeling approach.



The Team

team photo

Abstract

Notre projet on developing a novel treatment for hematological disorders caused by an iron overload, such as hemochromatosis and thalassemia. These autosomal recessive disorders have symptoms including cirrhosis, arthritis, and heart failure, which result from overabsorption of iron from the duodenum. Although these are among the most common heritable diseases, treatment options are limited. Even today patients are mostly treated by frequent bloodletting, which many people cannot support. The aim of our project is to combat these diseases at the source by developing a therapy that prevents the intestinal absorption of iron.

We engineer the Escherichia coli Ferric Uptake Regulation (FUR) system using a genetic inverter so that they produce siderophores (iron chelators) in response of high concentrations of iron. These engineered bacteria are delivered to the patient's intestine by encapsulating them in an ingestible polymer (capsule) that specifically degrades in the duodenum. Once released into the intestine, the bacteria respond to ambient iron by secreting elevated levels of siderophores, thereby chelating the iron to prevent its absorption by the patient.

Overview of the project and the achievements

Retrieved from "http://2013.igem.org/Team:Evry/vf"