Team:Bielefeld-Germany/Project/Cytochromes
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membrane in a way that it can transfer the electrons without causing its collapse. The integration of cytochromes into the | membrane in a way that it can transfer the electrons without causing its collapse. The integration of cytochromes into the | ||
membrane can help achieving a defined electron transport. A minimal set of cytochromes, consisting of the periplasmatic cytochrome MtrA, the outer membrane cytochrome MtrC and the outer membrane ß-barrel protein MtrB from ''Shewanella oneidensis'' MR-15 should be expressed in ''E. coli''. | membrane can help achieving a defined electron transport. A minimal set of cytochromes, consisting of the periplasmatic cytochrome MtrA, the outer membrane cytochrome MtrC and the outer membrane ß-barrel protein MtrB from ''Shewanella oneidensis'' MR-15 should be expressed in ''E. coli''. | ||
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+ | [[Image:Bielefeld-germany-project-overview-cytochromes.png|300px|thumb|center|'''Figure 1:''' Extracellular electron transfer via cytochromes in Shewanella oneidensins MR-1 with a minimal set of genes|]] | ||
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Revision as of 21:13, 18 September 2013
Mediators
Membranes can work as an isolator for the cell to prevent the transfer of charged molecules. So it is necessary to change the membrane in a way that it can transfer the electrons without causing its collapse. The integration of cytochromes into the membrane can help achieving a defined electron transport. A minimal set of cytochromes, consisting of the periplasmatic cytochrome MtrA, the outer membrane cytochrome MtrC and the outer membrane ß-barrel protein MtrB from ''Shewanella oneidensis'' MR-15 should be expressed in ''E. coli''.