"
Team:Evry/Notebook/w8
From 2013.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
We started the week by doing the golden gates over again, meaning the GG 3 and GG 2 (2.1 with FurBS 1, 2.2 with FurBS2 and 2.3 with FurBS3). In fact, our previous control positive had some unwanted white spots (01/08/13), thus suggesting some contaminations during the transformation step. curretnmy waiting | We started the week by doing the golden gates over again, meaning the GG 3 and GG 2 (2.1 with FurBS 1, 2.2 with FurBS2 and 2.3 with FurBS3). In fact, our previous control positive had some unwanted white spots (01/08/13), thus suggesting some contaminations during the transformation step. curretnmy waiting | ||
</p> | </p> | ||
| + | |||
| + | |||
| + | <p> | ||
| + | We performed 7 golden gates. | ||
| + | With prepared a mix for the 6 tubes : | ||
| + | <ul> | ||
| + | <li> 8,89 µl of 1A3 plasmide | ||
| + | <li> 12,18 µL of terminator | ||
| + | <li> 9,8 µL of T4 Buffer | ||
| + | <li> 3,5 µL of BSA | ||
| + | <li> 3,5 µL of T4 ligase | ||
| + | </ul> | ||
| + | </p> | ||
| + | <p> | ||
</br> | </br> | ||
Revision as of 10:12, 6 August 2013
Week 8: 5th August - 11th August
Plasmide 3:
We started the week by doing the golden gates over again, meaning the GG 3 and GG 2 (2.1 with FurBS 1, 2.2 with FurBS2 and 2.3 with FurBS3). In fact, our previous control positive had some unwanted white spots (01/08/13), thus suggesting some contaminations during the transformation step. curretnmy waiting
We performed 7 golden gates. With prepared a mix for the 6 tubes :
- 8,89 µl of 1A3 plasmide
- 12,18 µL of terminator
- 9,8 µL of T4 Buffer
- 3,5 µL of BSA
- 3,5 µL of T4 ligase
Additionnally, we did the optimization of our PCR over with the temperature gradient for the annealing step. current waiting
