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Revision as of 22:44, 6 August 2013 (view source) Sgrenz (Talk | contribs) ← Older edit		Revision as of 22:45, 6 August 2013 (view source) Sgrenz (Talk | contribs) Newer edit →
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	<col width="50">		<col width="50">
-	<tr> <th>Reagenz</th> <th>1x</th> <th>8</th> </tr>	+	<tr> <th>Reagenz</th> <th>1x</th> <th>8x</th> </tr>
	<tr> <td>Buffer 5x</td> <td>5,0</td> <td>44</td> </tr>		<tr> <td>Buffer 5x</td> <td>5,0</td> <td>44</td> </tr>
	<tr> <td>MgSO4</td> <td>0</td>      <td>0</td> </tr>		<tr> <td>MgSO4</td> <td>0</td>      <td>0</td> </tr>

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Revision as of 22:45, 6 August 2013

PCR Protocols and Programs

Phusion 25 µl

Reagenz	1x	8x
Buffer 5x	5,0	44
MgSO4	0	0
dNTPs	2,5	22
Primer1	1,0	8,8
Primer2	1,0	8,8
Template	1,5	13,2
DMSO	2,0	17,6
Polymerase	0,5	4,4
H2O	11,5	101,2