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Team:Bielefeld-Germany/Labjournal/Molecular
From 2013.igem.org
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Revision as of 23:03, 6 August 2013
PCR Protocols and Programs
Phusion 25 µl
| Reagenz | 1x | 8x |
|---|---|---|
| Buffer 5x | 5,0 | 44 |
| MgSO4 | 0 | 0 |
| dNTPs | 2,5 | 22 |
| Primer1 | 1,0 | 8,8 |
| Primer2 | 1,0 | 8,8 |
| Template | 1,5 | 13,2 |
| DMSO | 2,0 | 17,6 |
| Polymerase | 0,5 | 4,4 |
| H2O | 11,5 | 101,2 |
| Program Phusion | ||||
|---|---|---|---|---|
| 98 °C | 98 °C | x °C | 72 °C | 72 °C |
| 2 min | 30 s | 30 s | y | 6 min |
| x: according to Primer Tm y: 15s/kb | ||||
Taq 25 µl
| Reagenz | 1x | 8x |
|---|---|---|
| Buffer 5x | 5,0 | 44 |
| MgSO4 | 0 | 0 |
| dNTPs | 2,5 | 22 |
| Primer1 | 1,0 | 8,8 |
| Primer2 | 1,0 | 8,8 |
| Template | 1,5 | 13,2 |
| DMSO | 2,0 | 17,6 |
| Polymerase | 0,5 | 4,4 |
| H2O | 11,5 | 101,2 |
| Program Taq | ||||
|---|---|---|---|---|
| 98 °C | 98 °C | x °C | 72 °C | 72 °C |
| 2 min | 30 s | 30 s | y | 6 min |
| x: according to Primer Tm y: 15s/kb | ||||
