Team:Paris Saclay/Notebook/August/1

From 2013.igem.org

(Difference between revisions)
(Created page with "{{Team:Paris_Saclay/incl_debut_generique}} ='''Notebook : August 1'''= =='''Lab work'''== o ==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''=== ====''...")
Line 4: Line 4:
=='''Lab work'''==
=='''Lab work'''==
-
o
+
 
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''===
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''===
===='''1 - Gibson assembly.'''====
===='''1 - Gibson assembly.'''====
-
* BphR2 part1, BphR2 part2 and plasmid PSB1C3
+
* BphR2_part1, BphR2_part2 and plasmid PSB1C3
-
*_FNR_part1, FNR part1 and plasmid PSB1C3
+
* FNR_part1, FNR part1 and plasmid PSB1C3
-
* RBS FNR part1 and FNR_part2 and plasmid PSB1C3
+
* RBS_FNR part1 and FNR_part2 and plasmid PSB1C3
Protocol : [[Team:Paris_Saclay/Protocols/Gibson|PCR_clean_up]]
Protocol : [[Team:Paris_Saclay/Protocols/Gibson|PCR_clean_up]]
Line 22: Line 22:
*Epperdorf 1 : BphR2 part1(1ul), BphR2 part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
*Epperdorf 1 : BphR2 part1(1ul), BphR2 part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
*Epperdorf 2 : FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
*Epperdorf 2 : FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
-
*Epperdorf 3 : Bbs FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
+
*Epperdorf 3 : RBS_FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
|}
|}
{{Team:Paris_Saclay/incl_fin}}
{{Team:Paris_Saclay/incl_fin}}

Revision as of 06:00, 12 August 2013

Contents

Notebook : August 1

Lab work

A - Aerobic/Anaerobic regulation system / B - PCB sensing system

1 - Gibson assembly.

  • BphR2_part1, BphR2_part2 and plasmid PSB1C3
  • FNR_part1, FNR part1 and plasmid PSB1C3
  • RBS_FNR part1 and FNR_part2 and plasmid PSB1C3

Protocol : PCR_clean_up


Adding amount:

  • Epperdorf 1 : BphR2 part1(1ul), BphR2 part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
  • Epperdorf 2 : FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
  • Epperdorf 3 : RBS_FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)