Team:Paris Saclay/Notebook/August/1

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(Difference between revisions)
(→1 - Gibson assembly.)
(→1 - Gibson assembly.)
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==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''===
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''===
===='''1 - Gibson assembly.'''====
===='''1 - Gibson assembly.'''====
 +
Andy,Xiaojing
* BphR2_part1, BphR2_part2 and plasmid PSB1C3
* BphR2_part1, BphR2_part2 and plasmid PSB1C3
* FNR_part1, FNR part1 and plasmid PSB1C3
* FNR_part1, FNR part1 and plasmid PSB1C3
-
* RBS_FNR part1 and FNR_part2 and plasmid PSB1C3
+
* RBS_FNR part1, FNR_part2 and plasmid PSB1C3
Protocol : [[Team:Paris_Saclay/Protocols/Gibson|PCR_clean_up]]
Protocol : [[Team:Paris_Saclay/Protocols/Gibson|PCR_clean_up]]

Revision as of 06:28, 12 August 2013

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Contents

Notebook : August 1

Lab work

A - Aerobic/Anaerobic regulation system / B - PCB sensing system

1 - Gibson assembly.

Andy,Xiaojing

  • BphR2_part1, BphR2_part2 and plasmid PSB1C3
  • FNR_part1, FNR part1 and plasmid PSB1C3
  • RBS_FNR part1, FNR_part2 and plasmid PSB1C3

Protocol : PCR_clean_up

Sample Volume:

  • Epperdorf 1 : BphR2 part1(1ul), BphR2 part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
  • Epperdorf 2 : FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
  • Epperdorf 3 : RBS_FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)


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