Team:Paris Saclay/Notebook/August/1
From 2013.igem.org
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(→1 - Gibson assembly.) |
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* RBS_FNR part1, FNR_part2 and plasmid PSB1C3 | * RBS_FNR part1, FNR_part2 and plasmid PSB1C3 | ||
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Sample Volume: | Sample Volume: | ||
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- | * | + | *Tube 1 : BphR2 part1(1ul), BphR2 part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul) |
- | * | + | *Tube 2 : FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul) |
- | * | + | *Tube 3 : RBS_FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul) |
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+ | Put those 3 tubes into thermocycler at50℃ for 1 hour. | ||
+ | |||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} |
Revision as of 06:36, 12 August 2013
Contents |
Notebook : August 1
Lab work
A - Aerobic/Anaerobic regulation system / B - PCB sensing system
1 - Gibson assembly.
Andy,Xiaojing
- BphR2_part1, BphR2_part2 and plasmid PSB1C3
- FNR_part1, FNR part1 and plasmid PSB1C3
- RBS_FNR part1, FNR_part2 and plasmid PSB1C3
Sample Volume:
|
Put those 3 tubes into thermocycler at50℃ for 1 hour.