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Team:TU-Delft/Notebook/2013/08/19/
From 2013.igem.org
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<p> 1. Carried out colony PCR on pTET:RBS:cI and ran on gel. Colony 5 was selected and inoculated in LB Broth. <br> | <p> 1. Carried out colony PCR on pTET:RBS:cI and ran on gel. Colony 5 was selected and inoculated in LB Broth. <br> | ||
2. Carried out colony PCR on His-SUMO peptides with correct primer for pET23B and ran on gel. This gel gave good results. <br> | 2. Carried out colony PCR on His-SUMO peptides with correct primer for pET23B and ran on gel. This gel gave good results. <br> | ||
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| + | <img src="https://2013.igem.org/File:130819_ptet%2BcI.tif" height=300px> | ||
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3. Transformation of pBAD:AgrAC:pP2 was done in XL1 Blue cells and plated on agar plates. <br> | 3. Transformation of pBAD:AgrAC:pP2 was done in XL1 Blue cells and plated on agar plates. <br> | ||
4. PCR purification of His-SUMO-Peptides was done. <br> | 4. PCR purification of His-SUMO-Peptides was done. <br> | ||
5. 10X TBE buffer was made and kept in stock. <br> | 5. 10X TBE buffer was made and kept in stock. <br> | ||
</p> | </p> | ||
Revision as of 12:48, 22 August 2013
Notebook
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19th of August
Lab work
1. Carried out colony PCR on pTET:RBS:cI and ran on gel. Colony 5 was selected and inoculated in LB Broth.
2. Carried out colony PCR on His-SUMO peptides with correct primer for pET23B and ran on gel. This gel gave good results.
4. PCR purification of His-SUMO-Peptides was done.
5. 10X TBE buffer was made and kept in stock.
