From 2013.igem.org
(Difference between revisions)
Line 266:
Line 266: </tr>
</tr>
- <tr>
+
- <td rowspan="3" align="center">
+ <tr>
- <p>Fur Binding Site n°5
+ <td rowspan="6 " align="center">
- <br/>(with sfGFP)</p>
+ <p>Fep A
+ </p>
</td>
</td>
+
<td align="center">
<td align="center">
- 1
+ Fep A 2 Lac I
</td>
</td>
<td align="center">
<td align="center">
- 77.7 ng/µL
+ 50, 7
</td>
</td>
<td align="center">
<td align="center">
- 1.76
+ 14/08
</td>
</td>
<td align="center">
<td align="center">
- 0.99
+ No
</td>
</td>
</tr>
</tr>
+
+
<tr>
<tr>
<td align="center">
<td align="center">
- 3
+ Fep A 4 Lac I
</td>
</td>
<td align="center">
<td align="center">
- 78.4 ng/µL
+ 57, 4
</td>
</td>
<td align="center">
<td align="center">
- 1.61
+
</td>
</td>
<td align="center">
<td align="center">
- 0.81
+ No
</td>
</td>
</tr>
</tr>
+
+
<tr>
<tr>
<td align="center">
<td align="center">
- 4
+ Fep A 1 sfGFP
</td>
</td>
<td align="center">
<td align="center">
- 72.7 ng/µL
+
</td>
</td>
<td align="center">
<td align="center">
- 1.85
+ 05/08
</td>
</td>
<td align="center">
<td align="center">
- 4.23
+ No
</td>
</td>
</tr>
</tr>
<tr>
<tr>
- <td rowspan="4" align="center">
- <p>Fur Binding Site n°6
- <br/>(with sfGFP)</p>
- </td>
<td align="center">
<td align="center">
- 1
+ FepA 2 sfGFP
</td>
</td>
<td align="center">
<td align="center">
- 70.4 ng/µL
+
</td>
</td>
<td align="center">
<td align="center">
- 1.76
+ 05/08
</td>
</td>
<td align="center">
<td align="center">
- 2.16
+ No
</td>
</td>
</tr>
</tr>
+
<tr>
<tr>
<td align="center">
<td align="center">
- 2
- </td>
- <td align="center">
- 91.5 ng/µL
- </td>
- <td align="center">
- 1.55
- </td>
- <td align="center">
- 1.21
- </td>
- </tr>
- <tr>
- <td align="center">
- 3
- </td>
- <td align="center">
- 65.4 ng/µL
- </td>
- <td align="center">
- 1.70
- </td>
- <td align="center">
- 1.52
- </td>
- </tr>
- <tr>
- <td align="center">
- 4
- </td>
- <td align="center">
- 85.6 ng/µL
- </td>
- <td align="center">
- 1.72
- </td>
- <td align="center">
- 1.65
- </td>
- </tr>
- <tr>
- <td rowspan="3" align="center">
- <p>Fur Binding Site n°7
- <br/>(with sfGFP)</p>
</td>
</td>
<td align="center">
<td align="center">
- 1
- </td>
- <td align="center">
- 53.5 ng/µL
- </td>
- <td align="center">
- 1.76
- </td>
- <td align="center">
- 1.93
- </td>
- </tr>
- <tr>
- <td align="center">
- 2
- </td>
- <td align="center">
- 77.6 ng/µL
- </td>
- <td align="center">
- 1.69
- </td>
- <td align="center">
- 1.22
- </td>
- </tr>
- <tr>
- <td align="center">
- 3
- </td>
- <td align="center">
- 72.3 ng/µL
- </td>
- <td align="center">
- 1.68
- </td>
- <td align="center">
- 1.22
- </td>
- </tr>
- <tr>
- <td rowspan="4" align="center">
- <p>Fur Binding Site n°8
- <br/>(with sfGFP)</p>
</td>
</td>
<td align="center">
<td align="center">
- 1
- </td>
- <td align="center">
- 73.9 ng/µL
- </td>
- <td align="center">
- 1.58
- </td>
- <td align="center">
- 0.84
- </td>
- </tr>
- <tr>
- <td align="center">
- 2
- </td>
- <td align="center">
- 51.3 ng/µL
- </td>
- <td align="center">
- 1.75
- </td>
- <td align="center">
- 1.10
- </td>
- </tr>
- <tr>
- <td align="center">
- 3
- </td>
- <td align="center">
- 45.9 ng/µL
- </td>
- <td align="center">
- 1.85
- </td>
- <td align="center">
- 1.43
- </td>
- </tr>
- <tr>
- <td align="center">
- 4
- </td>
- <td align="center">
- 46.1 ng/µL
- </td>
- <td align="center">
- 1.88
- </td>
- <td align="center">
- 1.61
- </td>
- </tr>
- <tr>
- <td rowspan="4" align="center">
- <p>Fur Binding Site n°9
- <br/>(with sfGFP)</p>
</td>
</td>
<td align="center">
<td align="center">
- 1
- </td>
- <td align="center">
- 108.3 ng/µL
- </td>
- <td align="center">
- 1.78
- </td>
- <td align="center">
- 1.37
- </td>
- </tr>
- <tr>
- <td align="center">
- 2
- </td>
- <td align="center">
- 67.0 ng/µL
- </td>
- <td align="center">
- 1.88
- </td>
- <td align="center">
- 1.61
- </td>
- </tr>
- <tr>
- <td align="center">
- 3
- </td>
- <td align="center">
- 103.5 ng/µL
- </td>
- <td align="center">
- 1.87
- </td>
- <td align="center">
- 1.79
- </td>
- </tr>
- <tr>
- <td align="center">
- 4
- </td>
- <td align="center">
- 28.2 ng/µL
- </td>
- <td align="center">
- 2.06
- </td>
- <td align="center">
- 1.76
- </td>
- </tr>
- <tr>
- <td rowspan="4" align="center">
- <p>Fur Binding Site n°10
- <br/>(with sfGFP)</p>
- </td>
- <td align="center">
- 1
- </td>
- <td align="center">
- 29.5 ng/µL
- </td>
- <td align="center">
- 1.95
- </td>
- <td align="center">
- 1.22
- </td>
- </tr>
- <tr>
- <td align="center">
- 2
- </td>
- <td align="center">
- 70.0 ng/µL
- </td>
- <td align="center">
- 1.90
- </td>
- <td align="center">
- 1.67
- </td>
- </tr>
- <tr>
- <td align="center">
- 3
- </td>
- <td align="center">
- 46.0 ng/µL
- </td>
- <td align="center">
- 1.89
- </td>
- <td align="center">
- 1.62
- </td>
- </tr>
- <tr>
- <td align="center">
- 4
- </td>
- <td align="center">
- 55.8 ng/µL
- </td>
- <td align="center">
- 1.88
- </td>
- <td align="center">
- 1.44
</td>
</td>
</tr>
</tr>
<tr>
<tr>
- <td rowspan="4" align="center">
- <p>Fur Binding Site n°11
- <br/>(with sfGFP)</p>
- </td>
<td align="center">
<td align="center">
- 1
- </td>
- <td align="center">
- 55.3 ng/µL
- </td>
- <td align="center">
- 1.85
- </td>
- <td align="center">
- 1.49
- </td>
- </tr>
- <tr>
- <td align="center">
- 2
- </td>
- <td align="center">
- 87.1 ng/µL
- </td>
- <td align="center">
- 1.81
- </td>
- <td align="center">
- 1.37
- </td>
- </tr>
- <tr>
- <td align="center">
- 3
- </td>
- <td align="center">
- 57.7 ng/µL
- </td>
- <td align="center">
- 1.84
- </td>
- <td align="center">
- 1.44
- </td>
- </tr>
- <tr>
- <td align="center">
- 4
- </td>
- <td align="center">
- 21.7 ng/µL
- </td>
- <td align="center">
- 2.06
- </td>
- <td align="center">
- 1.20
- </td>
- </tr>
- <tr>
- <td rowspan="3" align="center">
- <p>Fur Binding Site n°15
- <br/>(with sfGFP)</p>
</td>
</td>
<td align="center">
<td align="center">
- 1
- </td>
- <td align="center">
- 16.2 ng/µL
- </td>
- <td align="center">
- 2.13
- </td>
- <td align="center">
- 1.20
- </td>
- </tr>
- <tr>
- <td align="center">
- 3
- </td>
- <td align="center">
- 47.4 ng/µL
- </td>
- <td align="center">
- 1.78
- </td>
- <td align="center">
- 1.16
- </td>
- </tr>
- <tr>
- <td align="center">
- 4
- </td>
- <td align="center">
- 63.2 ng/µL
- </td>
- <td align="center">
- 1.78
- </td>
- <td align="center">
- 1.16
- </td>
- </tr>
- </tbody>
- </table>
- <p>
- <b>Note:</b> Fur Binding Sites n°4, 12, 13 and 14 are missing.
- </p>
-
- <h2>3<sup>rd</sup> construction</h2>
-
- <h3>Sequencing preparation</h3>
-
- <p>
- Preculture with LB medium (with carbenicillin) have been launched for Top10 transformed with our plasmid which contains the sfGFP under the control of Lac O promoter. After one night of culture minipreps of 3 different clones have been realized using the miniprep kit from Machrey Nagel.
- </p>
-
- <table cellpadding="10" cellspacing="0" align='center' border="1">
- <thead>
- <tr>
- <th align="center">
- Clone
- </th>
- <th align="center">
- Concentration
- </th>
- <th align="center">
- 260/280
- </th>
- <th align="center">
- 260/230
- </th>
- </tr>
- </thead>
- <tbody>
- <tr>
- <td align="center">
- 1
</td>
</td>
<td align="center">
<td align="center">
- 25.7 ng/µL
- </td>
- <td align="center">
- 2.02
- </td>
- <td align="center">
- 2.29
- </td>
- </tr>
- <tr>
- <td align="center">
- 2
</td>
</td>
<td align="center">
<td align="center">
- 27.0 ng/µL
- </td>
- <td align="center">
- 2.09
- </td>
- <td align="center">
- 1.78
- </td>
- </tr>
- <tr>
- <td align="center">
- 3
- </td>
- <td align="center">
- 79.7 ng/µL
- </td>
- <td align="center">
- 1.86
- </td>
- <td align="center">
- 1.60
</td>
</td>
</tr>
</tr>
- </body>
- </table>
</div>
</div>
Revision as of 10:25, 20 August 2013
Iron coli project
Natural Promotor
Miniprep
Concentration (ng/µL)
Date
Biobricks
Ace B
AceB 1 Lac I
66,4
14/08
No
AceB 2 Lac I
53,8
14/08
No
Ace 4 Lac I
64,9
14/08
No
Ace B 1 sfGFP
166,1
05/08
No
Ace B 2 sfGFP
192,0
05/08
No
Ent C
Fec A
Fec A 1 sfGFP
05/08
No
Fec C
Fep A
Fep A 2 Lac I
50,7
14/08
No
Fep A 4 Lac I
57,4
No
Fep A 1 sfGFP
05/08
No
FepA 2 sfGFP
05/08
No
"
