Team:Evry/Protocols/07

From 2013.igem.org

(Difference between revisions)
Line 14: Line 14:
<p>Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence. <br>
<p>Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence. <br>
PCR is divided into 5 steps:<br>
PCR is divided into 5 steps:<br>
-
<ul><li>First denaturation<br>
+
First denaturation<br>
-
<li>Denaturation step<br>
+
Denaturation step<br>
-
<li>Anealing step<br>
+
Anealing step<br>
-
<li>Elongation step<br>
+
Elongation step<br>
-
<li>Final step<br></ul><br>
+
Final step<br>
The 2nd, 3th and 4th steps are repeated 20-40 cycles.<br></p>
The 2nd, 3th and 4th steps are repeated 20-40 cycles.<br></p>
 +
Denaturation step

Revision as of 16:24, 21 August 2013

Iron coli project

PCR and gel electrophoresis analysis

PCR

Principle

Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence.
PCR is divided into 5 steps:
First denaturation
Denaturation step
Anealing step
Elongation step
Final step
The 2nd, 3th and 4th steps are repeated 20-40 cycles.

Denaturation step

Preparation

Optimisation

Number of primer

Temperature

Gel electrophoresis analysis

Principle

Preparation

Analysis