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Team:CU-Boulder/Notebook/Protocols
From 2013.igem.org
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/EmbeddingPDF">Embedding PDF Files</a> <a href="https://static.igem.org/mediawiki/2013/9/99/Embedding_a_PDF_file_on_the_iGEM_Team_Websites.pdf"><i class="icon-download"></i></a></dt> | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/EmbeddingPDF">Embedding PDF Files</a> <a href="https://static.igem.org/mediawiki/2013/9/99/Embedding_a_PDF_file_on_the_iGEM_Team_Websites.pdf"><i class="icon-download"></i></a></dt> | ||
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<dd>In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.</dd> | <dd>In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.</dd> | ||
</dl> | </dl> | ||
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/GoogleDocEmbed">Embedding Google Docs</a> <a href="https://static.igem.org/mediawiki/2013/0/04/Embedding_Google_Docs_on_the_Wiki_-_Google_Drive.pdf"><i class="icon-download"></i></a></dt> | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/GoogleDocEmbed">Embedding Google Docs</a> <a href="https://static.igem.org/mediawiki/2013/0/04/Embedding_Google_Docs_on_the_Wiki_-_Google_Drive.pdf"><i class="icon-download"></i></a></dt> | ||
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<dd>Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.</dd> | <dd>Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.</dd> | ||
</dl> | </dl> | ||
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicallyCompetentCells">Chemically Competent Cells</a> <a href="https://static.igem.org/mediawiki/2013/d/da/Chemically_Competent_Cells.pdf"><i class="icon-download"></i></a></dt> | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicallyCompetentCells">Chemically Competent Cells</a> <a href="https://static.igem.org/mediawiki/2013/d/da/Chemically_Competent_Cells.pdf"><i class="icon-download"></i></a></dt> | ||
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<dd>This protocol details how to prepare chemically competent cells.</dd> | <dd>This protocol details how to prepare chemically competent cells.</dd> | ||
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicalTransformations">Chemical Transformations</a> <a href="https://static.igem.org/mediawiki/2013/1/15/Chemical_Transformations.pdf"><i class="icon-download"></i></a></dt> | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicalTransformations">Chemical Transformations</a> <a href="https://static.igem.org/mediawiki/2013/1/15/Chemical_Transformations.pdf"><i class="icon-download"></i></a></dt> | ||
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<dd>In this protocol we cover how to use our chemically competent cells to perform transformations.</dd> | <dd>In this protocol we cover how to use our chemically competent cells to perform transformations.</dd> | ||
</dl> | </dl> | ||
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/PlasmidMinipreps">Plasmid Minipreps</a> <a href="https://static.igem.org/mediawiki/2013/0/08/Plasmid_Miniprep.pdf"><i class="icon-download"></i></a></dt> | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/PlasmidMinipreps">Plasmid Minipreps</a> <a href="https://static.igem.org/mediawiki/2013/0/08/Plasmid_Miniprep.pdf"><i class="icon-download"></i></a></dt> | ||
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<dd>Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.</dd> | <dd>Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.</dd> | ||
</dl> | </dl> | ||
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RestrictionDigests">Restriction (Double) Digests</a> <a href="https://static.igem.org/mediawiki/2013/f/f7/Restriction_Digests.pdf"><i class="icon-download"></i></a></dt> | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RestrictionDigests">Restriction (Double) Digests</a> <a href="https://static.igem.org/mediawiki/2013/f/f7/Restriction_Digests.pdf"><i class="icon-download"></i></a></dt> | ||
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<dd>Our method of performing restriction double digests is detailed in this protocol.</dd> | <dd>Our method of performing restriction double digests is detailed in this protocol.</dd> | ||
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/HomemadeBufferCompositions">Homemade Buffer Compositions</a> <a href="https://static.igem.org/mediawiki/2013/8/85/Homemade_Buffer_Compositions.pdf"><i class="icon-download"></i></a></dt> | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/HomemadeBufferCompositions">Homemade Buffer Compositions</a> <a href="https://static.igem.org/mediawiki/2013/8/85/Homemade_Buffer_Compositions.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <p></p> | ||
<dd>The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.</dd> | <dd>The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.</dd> | ||
</dl> | </dl> | ||
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RecyclingMinicolumns">Recycling DNA Mini-columns</a> <a href="https://static.igem.org/mediawiki/2013/d/dc/Recycling_DNA_Mini-columns.pdf"><i class="icon-download"></i></a></dt> | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RecyclingMinicolumns">Recycling DNA Mini-columns</a> <a href="https://static.igem.org/mediawiki/2013/d/dc/Recycling_DNA_Mini-columns.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <p></p> | ||
<dd>This protocol covers how to recycle mini-columns used in plasmid miniprep systems.</dd> | <dd>This protocol covers how to recycle mini-columns used in plasmid miniprep systems.</dd> | ||
</dl> | </dl> | ||
Revision as of 16:46, 22 August 2013
- Embedding PDF Files
- In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.
- Embedding Google Docs
- Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.
- Chemically Competent Cells
- This protocol details how to prepare chemically competent cells.
- Chemical Transformations
- In this protocol we cover how to use our chemically competent cells to perform transformations.
- Plasmid Minipreps
- Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.
- Restriction (Double) Digests
- Our method of performing restriction double digests is detailed in this protocol.
- Homemade Buffer Compositions
- The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.
- Recycling DNA Mini-columns
- This protocol covers how to recycle mini-columns used in plasmid miniprep systems.
