Team:Evry/Protocols/02
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(Difference between revisions)
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- | <h1> | + | <h1> Transformation </h1> |
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<h2> Preparation </h2> | <h2> Preparation </h2> | ||
- | <p>For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. | + | <p>For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. If the ligation protocol is a |
+ | <a href="https://2013.igem.org/Team:Evry/Protocols/05">Golden Gate</a> , add 5 µL instead.<br/> | ||
Let 30 minutes on ice.<br/> | Let 30 minutes on ice.<br/> | ||
Let the cells at 42°C for exactly 50 seconds.<br/> | Let the cells at 42°C for exactly 50 seconds.<br/> | ||
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Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.<br/> | Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.<br/> | ||
</p> | </p> | ||
- | <h2> | + | <h2> Tests</h2> |
- | + | ||
Revision as of 10:51, 26 August 2013
Transformation
Principle
Preparation
For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. If the ligation protocol is a
Golden Gate , add 5 µL instead.
Let 30 minutes on ice.
Let the cells at 42°C for exactly 50 seconds.
Let 5 minutes on ice.
Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.
Tests