"
Team:Evry/Protocols/07
From 2013.igem.org
(Difference between revisions)
| Line 18: | Line 18: | ||
-First denaturation<br> | -First denaturation<br> | ||
-Denaturation step<br> | -Denaturation step<br> | ||
| - | - | + | -Annealing step<br> |
-Elongation step<br> | -Elongation step<br> | ||
-Final step<br> | -Final step<br> | ||
Revision as of 12:45, 26 August 2013
PCR
Principle
Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence.
PCR is divided into 5 steps:
-First denaturation
-Denaturation step
-Annealing step
-Elongation step
-Final step
The 2nd, 3th and 4th steps are repeated 20-40 cycles.
Preparation
Optimisation
Number of primer
Temperature
Gel electrophoresis analysis
Principle
Preparation
Analysis
Site developped by Gabriel Guillocheau
