Team:Evry/Protocols/07

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<h1> PCR </h1>
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<h1> Polymerase Chain Reaction </h1>
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<h2> Principle</h2>
<h2> Principle</h2>
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<p>Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence. <br>
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<p>Polymerase Chain Reaction (PCR) is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence. <br>
PCR is divided into 5 steps:<br>
PCR is divided into 5 steps:<br>
   - First denaturation<br>
   - First denaturation<br>

Revision as of 12:47, 26 August 2013

Iron coli project

Polymerase Chain Reaction

PCR

Principle

Polymerase Chain Reaction (PCR) is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence.
PCR is divided into 5 steps:
- First denaturation
- Denaturation step
- Annealing step
- Elongation step
- Final step
The 2nd, 3th and 4th steps are repeated 20-40 cycles.

Denaturation step

Preparation

Optimisation

Number of primer

Temperature

Gel electrophoresis analysis

Principle

Preparation

Analysis

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