Notebook : July 11

Summary:

For régulation system:

• 1.For those transformation products, RBS+LacS+terminator in PSB1C3 plasmid, the liquid culture has been performed for further experiments. The oligopetides for PCR amplification was made bioinformaticly. The transformation for the terminator BBa_B0010 was still fail.
• 2. The plasmid which contains fnr+RBS+LacZ+terminator and fnr+RBS+AmilCP+terminator was extracted. The restiction digest was performer for them.

For sensor system:

• 3. The selection of bacterian colonies was achieved by analyzing PCR product. The baterian selected are BphR1 c5 and c6; BphR2 c3 and c4; BphA1 c5 and c6,c7,c8.

Lab work

Verification by digestion Simple DNA 3µl Buffer 3 µl Enzyme(Xho I or Sac II) 1µl H2O 23µl Total:30µl Double DNA 5µl Buffer 3 µl Enzyme(Xho I or Sac II) 2µl H2O 20µl Total:30µl

Buffer : Ecor I+ PST I -> orange XhoI->green Sac I->blue xhoI+Sac II-> green

size estimed Ecor I+PST I : 1069bp and 2750 bp Xho I : 2976bp+842bp Sac II:3819bp Xho I+Sac II: 843bp,616bp,2367bp Size observed Ecor I+PST I : 2700bp and 1000bp Xho I : 4000bp Sac II:4000bp Xho I+Sac II: 1200bp and 2000bp