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Team:Evry/Protocols/11
From 2013.igem.org
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| + | Add 1:1 volume of Binding Buffer to completed PCR mixture (e.g. if you have 100 µL of reaction mixture, add 100 µL of Binding Buffer). | ||
| + | Mix thoroughly and check the solution's color: a yellow color color indicates an optimal pH for DNA binding. | ||
<br><br></p> | <br><br></p> | ||
Revision as of 13:09, 29 August 2013
PCR Purification
Goal
The aim of the PCRpurification step is to recover DNA from PCR and other enzymatic reacti mixtures.
Preparation
Protocol adapted from Thermo Scientific PCR Purification notebook1.
Add 1:1 volume of Binding Buffer to completed PCR mixture (e.g. if you have 100 µL of reaction mixture, add 100 µL of Binding Buffer).
Mix thoroughly and check the solution's color: a yellow color color indicates an optimal pH for DNA binding.
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Test
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