Team:TU-Munich/Team/Attributions
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The research group at the [http://biologische-chemie.userweb.mwn.de/index.html Chair of Biological Chemistry] at TUM works in the biochemical field of protein engineering and design with its objectives set on the construction of artifical proteins with novel functions through rational as well as deductive research approaches. In this context, a range of methods facilitating the functional and structural analysis of native proteins was developed over the last years, with an increasing role of protein crystallography. | The research group at the [http://biologische-chemie.userweb.mwn.de/index.html Chair of Biological Chemistry] at TUM works in the biochemical field of protein engineering and design with its objectives set on the construction of artifical proteins with novel functions through rational as well as deductive research approaches. In this context, a range of methods facilitating the functional and structural analysis of native proteins was developed over the last years, with an increasing role of protein crystallography. | ||
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+ | ''Prof. Skerra kindly provided us with space in his laboratory, and generously advanced us money to loan to pay for team registration, travel expenses and laboratory resources. Moreover he visited our team meeting and gave advice on our project.'' | ||
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==== Laboratory of Prof. Dr. Helmreich ==== | ==== Laboratory of Prof. Dr. Helmreich ==== | ||
- | [http://www.sww.bv.tum.de Sanitary Environmental Engineering] is a horizontal discipline comprised of civil engineering, process engineering and chemistry/biology. Research and teaching include the fields of water suppy, sewage and rain water treatment, water quality and the modelling of aquatic systems | + | [http://www.sww.bv.tum.de Sanitary Environmental Engineering] is a horizontal discipline comprised of civil engineering, process engineering and chemistry/biology. Research and teaching include the fields of water suppy, sewage and rain water treatment, water quality and the modelling of aquatic systems. |
''In our interview with Prof. Helmreich, she explained us several characterization techniques to assess water quality. Furthermore she kindly provided us with water samples from sewage treatment plants.'' | ''In our interview with Prof. Helmreich, she explained us several characterization techniques to assess water quality. Furthermore she kindly provided us with water samples from sewage treatment plants.'' | ||
==== Laboratory of Prof. Dr. Langosch ==== | ==== Laboratory of Prof. Dr. Langosch ==== | ||
- | The [http://www.wzw.tum.de/biopolymere Chair for Chemistry of Biopolymers] focuses on the structural biochemistry of integral membrane proteins. Core themes are molecular interactions between membrane proteins, strucural dynamics of membrane bound protein helices, membrane protein/lipid interactions, structure/function relationships of integral membrane protein complexes | + | The [http://www.wzw.tum.de/biopolymere Chair for Chemistry of Biopolymers] focuses on the structural biochemistry of integral membrane proteins. Core themes are molecular interactions between membrane proteins, strucural dynamics of membrane bound protein helices, membrane protein/lipid interactions, structure/function relationships of integral membrane protein complexes. |
- | ''During the planning phase of our project we asked Prof. Langosch for advice concerning the design of the transmembrane | + | ''During the planning phase of our project we asked Prof. Langosch for advice concerning the design of the transmembrane domain of our constructs.'' |
- | ==== Laboratory of Prof. Dr. | + | ==== Laboratory of Prof. Dr. Rost ==== |
+ | TEXT | ||
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+ | ''After developing a prototype version of our AutoAnnotator [LINK HERE], we presented our work to Prof. Rost. He gave us some advice what features we could add and provided us access to his ProteinPredict [LINK HERE] software server.'' | ||
==== Laboratory of Prof. Dr. Schwechheimer ==== | ==== Laboratory of Prof. Dr. Schwechheimer ==== | ||
- | The [http://www.sysbiol.wzw.tum.de Chair for Systems Biology of Plants] investigates a range of queries concerning the ubiquitin proteasome system of plants applying a combination of genetics, molecular biology and cell biology with both genomic and proteomic approaches | + | The [http://www.sysbiol.wzw.tum.de Chair for Systems Biology of Plants] investigates a range of queries concerning the ubiquitin proteasome system of plants applying a combination of genetics, molecular biology and cell biology with both genomic and proteomic approaches. |
- | ''Together with Prof. Schwechheimer we discussed several different signal transduction pathways that could be utilised for our kill-switch. Moreover he kindly granted us access to a fluorescence microscope.'' | + | ''Together with Prof. Schwechheimer we discussed several different signal transduction pathways that could be utilised for our kill-switch [LINK HERE]. Moreover he kindly granted us access to a fluorescence microscope.'' |
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==== Laboratory of Prof. Dr. Reski at Freiburg University ==== | ==== Laboratory of Prof. Dr. Reski at Freiburg University ==== | ||
- | The team at the [http://www.plant-biotech.net/ Chair for Plant Biotechnology] is working on gene expression | + | The team at the [http://www.plant-biotech.net/ Chair for Plant Biotechnology] is working on gene expression in the bryophyte model plant ''Physcomitrella patens'' (Hedw.) B.S.G. at different levels in correlation with phenotype analysis and additionally employs comparative genomics approaches. |
''We would especially like to thank Dr. Gertrud Wiedemann, who kindly took the time and patience to teach us the protoplast transfection method at her lab. Also a big thank you to Omar Saleh, who helped us with the osmometer and to Anja Kuberski.'' | ''We would especially like to thank Dr. Gertrud Wiedemann, who kindly took the time and patience to teach us the protoplast transfection method at her lab. Also a big thank you to Omar Saleh, who helped us with the osmometer and to Anja Kuberski.'' | ||
==== Laboratory of Prof. Dr. Fussenegger at ETH Zurich ==== | ==== Laboratory of Prof. Dr. Fussenegger at ETH Zurich ==== | ||
- | The research group at the [http://www.bsse.ethz.ch/groups/group_fussenegger/index/ Chair for Biotechnology and Bioengineering] is implementing progress in basic research to achieve generic and prototypic advances in human therapy by focusing on mammalian cells and capitalizing on an integrated interdisciplinary systems approach | + | The research group at the [http://www.bsse.ethz.ch/groups/group_fussenegger/index/ Chair for Biotechnology and Bioengineering] is implementing progress in basic research to achieve generic and prototypic advances in human therapy by focusing on mammalian cells and capitalizing on an integrated interdisciplinary systems approach. |
''They kindly provided us with the pSH21 (IRES) plasmid.'' | ''They kindly provided us with the pSH21 (IRES) plasmid.'' | ||
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''Kindly provided us with the plasmids pDEST14_ereA and pDEST14_ereB.'' | ''Kindly provided us with the plasmids pDEST14_ereA and pDEST14_ereB.'' | ||
- | ==== Laboratory of Prof. Dr. Arndt | + | ==== Laboratory of Prof. Dr. Arndt at Potsdam University) ==== |
+ | The team at the [http://www.uni-potsdam.de/index.php?id=13895 Chair for Molecular Biotechnology] ... | ||
''Sven Hagen kindly provided us with the plasmids pBad-mVenus (RFC 25).'' | ''Sven Hagen kindly provided us with the plasmids pBad-mVenus (RFC 25).'' |
Revision as of 14:25, 6 September 2013
Laboratory of Prof. Dr. Skerra
The research group at the [http://biologische-chemie.userweb.mwn.de/index.html Chair of Biological Chemistry] at TUM works in the biochemical field of protein engineering and design with its objectives set on the construction of artifical proteins with novel functions through rational as well as deductive research approaches. In this context, a range of methods facilitating the functional and structural analysis of native proteins was developed over the last years, with an increasing role of protein crystallography.
Prof. Skerra kindly provided us with space in his laboratory, and generously advanced us money to loan to pay for team registration, travel expenses and laboratory resources. Moreover he visited our team meeting and gave advice on our project.
Technical University Munich
Laboratory of Prof. Dr. Helmreich
[http://www.sww.bv.tum.de Sanitary Environmental Engineering] is a horizontal discipline comprised of civil engineering, process engineering and chemistry/biology. Research and teaching include the fields of water suppy, sewage and rain water treatment, water quality and the modelling of aquatic systems.
In our interview with Prof. Helmreich, she explained us several characterization techniques to assess water quality. Furthermore she kindly provided us with water samples from sewage treatment plants.
Laboratory of Prof. Dr. Langosch
The [http://www.wzw.tum.de/biopolymere Chair for Chemistry of Biopolymers] focuses on the structural biochemistry of integral membrane proteins. Core themes are molecular interactions between membrane proteins, strucural dynamics of membrane bound protein helices, membrane protein/lipid interactions, structure/function relationships of integral membrane protein complexes.
During the planning phase of our project we asked Prof. Langosch for advice concerning the design of the transmembrane domain of our constructs.
Laboratory of Prof. Dr. Rost
TEXT
After developing a prototype version of our AutoAnnotator [LINK HERE], we presented our work to Prof. Rost. He gave us some advice what features we could add and provided us access to his ProteinPredict [LINK HERE] software server.
Laboratory of Prof. Dr. Schwechheimer
The [http://www.sysbiol.wzw.tum.de Chair for Systems Biology of Plants] investigates a range of queries concerning the ubiquitin proteasome system of plants applying a combination of genetics, molecular biology and cell biology with both genomic and proteomic approaches.
Together with Prof. Schwechheimer we discussed several different signal transduction pathways that could be utilised for our kill-switch [LINK HERE]. Moreover he kindly granted us access to a fluorescence microscope.
Other Universities
Laboratory of Prof. Dr. Reski at Freiburg University
The team at the [http://www.plant-biotech.net/ Chair for Plant Biotechnology] is working on gene expression in the bryophyte model plant Physcomitrella patens (Hedw.) B.S.G. at different levels in correlation with phenotype analysis and additionally employs comparative genomics approaches.
We would especially like to thank Dr. Gertrud Wiedemann, who kindly took the time and patience to teach us the protoplast transfection method at her lab. Also a big thank you to Omar Saleh, who helped us with the osmometer and to Anja Kuberski.
Laboratory of Prof. Dr. Fussenegger at ETH Zurich
The research group at the [http://www.bsse.ethz.ch/groups/group_fussenegger/index/ Chair for Biotechnology and Bioengineering] is implementing progress in basic research to achieve generic and prototypic advances in human therapy by focusing on mammalian cells and capitalizing on an integrated interdisciplinary systems approach.
They kindly provided us with the pSH21 (IRES) plasmid.
Laboratory of Dr. G.D. Wright at McMaster University
The [http://www.thewrightlab.com Wright Lab] is trying to understand fundamental aspects of how antibiotics work, their sources and how bacteria become resistant to them.
Kindly provided us with the plasmids pDEST14_ereA and pDEST14_ereB.
Laboratory of Prof. Dr. Arndt at Potsdam University)
The team at the [http://www.uni-potsdam.de/index.php?id=13895 Chair for Molecular Biotechnology] ...
Sven Hagen kindly provided us with the plasmids pBad-mVenus (RFC 25).
Webdesign
Software
James Padolsey ([http://james.padolsey.com see his website])
We used his jQuery extension for [http://james.padolsey.com/javascript/cross-domain-requests-with-jquery/ Cross-domain AJAX requests] in the AutoAnnotator.
AutoAnnotator:
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Email: igem@wzw.tum.de
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