Team:Evry/Primers/vf

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Revision as of 09:23, 9 September 2013

Iron coli project

Primers

CODE NOM Taille Ta SÉQUENCE

P001

Primer Fur
(Forward - GoldenBrick sequence)

41 nt

74°C

CGTGAATTCGGTCTCTCTAGATGACTGATAACAATACCGCC

P002

Primer Fur
(Reverse - GoldenBrick sequence)

48 nt

77°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGCCTTCGTGCG

P005

RBS B0034
(Forward - GoldenBrick sequence)

47 nt

75°C

GAATTCGGTCTCTTGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA

P006

RBS B0034
(Reverse - GoldenBrick sequence)

47 nt

75°C

TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCAAGAGACCGAATTC

P007

aceB Natural Fur promoter
(Forward - GoldenBrick sequence)

42 nt

76°C

GAATTCGGTCTCAGCCTCTAGAGATCTACGGCACATGAATCC

P008

ace B Natural Fur promoter
(Reverse - GoldenBrick sequence)

32 nt

78°C

TGCAGGGTCTCACTAGTACGTGCAGCTCCTCG

P009

Ent C Natural Fur promoter
(Forward - GoldenBrick sequence)

49 nt

73°C

GAATTCGGTCTCAGCCTCTAGAGAAATCAGCTTCCTGTTATTAATAAGG

P010

Ent C Natural Fur promoter
(Reverse - GoldenBrick sequence)

32 nt

64°C

ACTAGTAATCATCCTCCACAAAATGATAAAGG

P011

Fec A Natural Fur promoter
(Forward - GoldenBrick sequence)

50 nt

73°C

GAATTCGGTCTCAGCCTCTAGAGGGAAAATAATTCTTATTTCGATTGTCC

P012

Fec A Natural Fur promoter
(Reverse - GoldenBrick sequence)

41 nt

74°C

CTGCAGGGTCTCACTAGTAACCTTCCCCATCATCATTTTTG

P013

Fec C Natural Fur promoter
(Forward - GoldenBrick sequence)

42 nt

77°C

GAATTCGGTCTCAGCCTCTAGAGGAACAGCAATTCAACCTGC

P014

Fec C Natural Fur promoter
(Reverse - GoldenBrick sequence)

36 nt

75°C

CTGCAGGGTCTCACTAGTATTCACAACGGTAAGCGG

P015

Andersen's promoter J23100
(Forward - GoldenBrick sequence)

70 nt

84°C

GAATTCGGTCTCAGCCTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCGTGATGAGACCG

P016

Andersen's promoter J23100
(Reverse - GoldenBrick sequence)

70 nt

84°C

CGGTCTCATCACGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAGGCTGAGACCGAATTC

P017

Fur Binding Site N°1
(Foward - GoldenBrick sequence)

45 nt

72°C

CGGTCTCTGTGAATTATTGATAACTATTTGGATGCTAGAGACCAC

P018

Fur Binding Site N°1
(Reverse - GoldenBrick sequence)

45 nt

72°C

GTGGTCTCTAGCATCCAAATAGTTATCAATAATTCACAGAGACCG

P019

Fur Binding Site N°2
(Foward - GoldenBrick sequence)

47 nt

75°C

CGGTCTCTGTGAGATAACTATTTGCATTTGCAGATGCTAGAGACCAC

P020

Fur Binding Site N°2
(Reverse - GoldenBrick sequence)

47 nt

75°C

GTGGTCTCTAGCATCTGCAAATGCAAATAGTTATCTCACAGAGACCG

P021

Fep A Natural Fur promoter
(Foward - GoldenBrick sequence)

41 nt

76°C

GAATTCGGTCTCAGCCTCTAGAGTTCGTCATTCAGACGCTG

P022

Fep A Natural Fur promoter
(Reverse - GoldenBrick sequence)

42 nt

73°C

CTGCAGGGTCTCACTAGTATGTTTTATTCCTGCATTTTTGCC

P023

Fes Natural Fur promoter
(Foward - GoldenBrick sequence)

40 nt

77°C

GAATTCGGTCTCAGCCTCTAGAGGCCACGAATTGCAACTG

P024

Fes Natural Fur promoter
(Reverse - GoldenBrick sequence)

37 nt

75°C

CTGCAGGGTCTCACTAGTATTCGTCATTCAGACGCTG

P025

Sdh C Natural Fur promoter
(Foward - GoldenBrick sequence)

45 nt

75°C

GAATTCGGTCTCAGCCTCTAGAGATCACTGTTCAGGATAAAACCC

P026

Sdh C Natural Fur promoter
(Reverse - GoldenBrick sequence)

36 nt

76°C

CTGCAGGGTCTCACTAGTAGAATAACGCCCACATGC

P027

ybi L Natural Fur promoter
(Forward - GoldenBrick sequence)

50 nt

76°C

GAATTCGGTCTCAGCCTCTAGAGCACCTAATTTGCCCATGATGATATCTC

P028

ybi L Natural Fur promoter
(Reverse - GoldenBrick sequence)

50 nt

72°C

CTGCAGGGTCTCACTAGTAGTGATGGCTTCTGATAAAGATAAATTTATAG

P029

ync E Natural Fur promoter
(Forward - GoldenBrick sequence)

40 nt

78°C

GAATTCGGTCTCAGCCTCTAGAGACGGGGGAAAGGACAAG

P030

ync E Natural Fur promoter
(Reverse - GoldenBrick sequence)

39 nt

75°C

CTGCAGGGTCTCACTAGTAGACGACTCCCTTTGATGAAC

P031

Fur Binding Site N°3
(Forward - GoldenBrick sequence)

47 nt

75°C

CGGTCTCTGTGACAAATGCAAATAGTTATCCAGATGCTAGAGACCAC

P032

Fur Binding Site N°3
(Reverse - GoldenBrick sequence)

47 nt

75°C

GTGGTCTCTAGCATCTGGATAACTATTTGCATTTGTCACAGAGACCG

P033

Fur Binding Site N°4
(Forward - GoldenBrick sequence)

47 nt

72°C

CGGTCTCTGTGACAAATAGTTATCAATAATCAGATGCTAGAGACCAC

P034

Fur Binding Site N°4
(Reverse - GoldenBrick sequence)

47 nt

72°C

GTGGTCTCTAGCATCTGATTATTGATAACTATTTGTCACAGAGACCG

P035

Fur Binding Site N°5
(Forward - GoldenBrick sequence)

47 nt

70°C

CGGTCTCTGTGAGTAAATTAATATTATTTACAGATGCTAGAGACCAC

P036

Fur Binding Site N°5
(Reverse - GoldenBrick sequence)

47 nt

70°C

GTGGTCTCTAGCATCTGTAAATAATATTAATTTACTCACAGAGACCG

P037

Fur Binding Site N°6
(Forward - GoldenBrick sequence)

47 nt

74°C

CGGTCTCTGTGAAATAATGCTTCTCATTTTCAGATGCTAGAGACCAC

P038

Fur Binding Site N°6
(Reverse - GoldenBrick sequence)

47 nt

74°C

GTGGTCTCTAGCATCTGAAAATGAGAAGCATTATTTCACAGAGACCG

P039

Fur Binding Site N°7
(Forward - GoldenBrick sequence)

47 nt

71°C

CGGTCTCTGTGAATAAATGATAATCATTATCAGATGCTAGAGACCAC

P040

Fur Binding Site N°7
(Reverse - GoldenBrick sequence)

47 nt

71°C

GTGGTCTCTAGCATCTGATAATGATTATCATTTATTCACAGAGACCG

P041

Fur Binding Site N°8
(Forward - GoldenBrick sequence)

47 nt

73°C

CGGTCTCTGTGAGAAAATAATTCTTATTTCCAGATGCTAGAGACCAC

P042

Fur Binding Site N°8
(Reverse - GoldenBrick sequence)

47 nt

73°C

GTGGTCTCTAGCATCTGGAAATAAGAATTATTTTCTCACAGAGACCG

P043

Fur Binding Site N°9
(Forward - GoldenBrick sequence)

47 nt

73°C

CGGTCTCTGTGAAGCACTTATTATTATTTTCAGATGCTAGAGACCAC

P044

Fur Binding Site N°9
(Reverse - GoldenBrick sequence)

47 nt

73°C

GTGGTCTCTAGCATCTGAAAATAATAATAAGTGCTTCACAGAGACCG

P045

Fur Binding Site N°10
(Forward - GoldenBrick sequence)

47 nt

75°C

CGGTCTCTGTGAGATAATTGTTATCGTTTGCAGATGCTAGAGACCAC

P046

Fur Binding Site N°10
(Reverse - GoldenBrick sequence)

47 nt

75°C

GTGGTCTCTAGCATCTGCAAACGATAACAATTATCTCACAGAGACCG

P047

Fur Binding Site N°11
(Forward - GoldenBrick sequence)

47 nt

74°C

CGGTCTCTGTGAGATAATGCTTATCAAAATCAGATGCTAGAGACCAC

P048

Fur Binding Site N°11
(Reverse - GoldenBrick sequence)

47 nt

74°C

GTGGTCTCTAGCATCTGATTTTGATAAGCATTATCTCACAGAGACCG

P049

Fur Binding Site N°12
(Forward - GoldenBrick sequence)

47 nt

73°C

CGGTCTCTGTGACAAAATTATTATCACTTTCAGATGCTAGAGACCAC

P050

Fur Binding Site N°12
(Reverse - GoldenBrick sequence)

47 nt

73°C

GTGGTCTCTAGCATCTGAAAGTGATAATAATTTTGTCACAGAGACCG

P051

Fur Binding Site N°13
(Forward - GoldenBrick sequence)

47 nt

75°C

CGGTCTCTGTGAAATAATGATTACCATTCCCAGATGCTAGAGACCAC

P052

Fur Binding Site N°13
(Reverse - GoldenBrick sequence)

47 nt

75°C

GTGGTCTCTAGCATCTGGGAATGGTAATCATTATTTCACAGAGACCG

P053

Fur Binding Site N°14
(Forward - GoldenBrick sequence)

47 nt

73°C

CGGTCTCTGTGAGAAATTGTTTTTGATTTTCAGATGCTAGAGACCAC

P054

Fur Binding Site N°14
(Reverse - GoldenBrick sequence)

47 nt

73°C

GTGGTCTCTAGCATCTGAAAATCAAAAACAATTTCTCACAGAGACCG

P055

Fur Binding Site N°15
(Forward - GoldenBrick sequence)

47 nt

73°C

CGGTCTCTGTGAGTTAATTGTAATGATTTTCAGATGCTAGAGACCAC

P056

Fur Binding Site N°15
(Reverse - GoldenBrick sequence)

47 nt

73°C

GTGGTCTCTAGCATCTGAAAATCATTACAATTAACTCACAGAGACCG

P057

Andersen's promoter J23101
(Forward - GoldenBrick sequence)

70 nt

81°C

GAATTCGGTCTCAGCCTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCGTGATGAGACCG

P058

Andersen's promoter J23101
(Reverse - GoldenBrick sequence)

70 nt

81°C

CGGTCTCATCACGCTAGCATAATACCTAGGACTGAGCTAGCTGTAAACTCTAGAGGCTGAGACCGAATTC

P059

Andersen's promoter J23102
(Forward - GoldenBrick sequence)

70 nt

83°C

GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTACTGTGCTAGCGTGATGAGACCG

P060

Andersen's promoter J23102
(Reverse - GoldenBrick sequence)

70 nt

83°C

CGGTCTCATCACGCTAGCACAGTACCTAGGACTGAGCTAGCTGTCAACTCTAGAGGCTGAGACCGAATTC

P061

Andersen's promoter J23104
(Forward - GoldenBrick sequence)

70 nt

82°C

GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTATTGTGCTAGCGTGATGAGACCG

P062

Andersen's promoter J23104
(Reverse - GoldenBrick sequence)

70 nt

82°C

CGGTCTCATCACGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAACTCTAGAGGCTGAGACCGAATTC

P063

VR primer iGEM standard

20 nt

63°C

ATTACCGCCTTTGAGTGAGC

P064

VF2 primer iGEM standard

20 nt

64°C

TGCCACCTGACGTCTAAGAA

P065

Ent A Biosynthesis of Enterobactin Siderophore
(Forward - GoldenBrick sequence)

74 nt

81°C

GAATTCGGTCTCTGTTCGGACCCATCTAGGTCCAGAGATAAGGAGGACAAACATGGATTTCAGCGGTAAAAATG

P066

Ent A Biosynthesis of Enterobactin Siderophore
(Reverse - GoldenBrick sequence)

45 nt

81°C

GGACTGCAGACTAGTGGTCTCTTGGATCTTATGCCCCCAGCGTTG

P067

Ent B Biosynthesis of Enterobactin Siderophore
(Forward - GoldenBrick sequence)

74 nt

78°C

GAATTCGGTCTCTGTTCACATACTAAACACATCAATCAAGGAGGTACTAGGATGGCTATTCCAAAATTACAGGC

P068

Ent B Biosynthesis of Enterobactin Siderophore
(Reverse - GoldenBrick sequence)

45 nt

79°C

GGACTGCAGACTAGTGGTCTCTTGGATCTTATTTCACCTCGCGGG

P069

Ent C Biosynthesis of Enterobactin Siderophore
(Forward - GoldenBrick sequence)

68 nt

80°C

GAATTCGGTCTCTTCCAATACTAAGCAGTTACAAGGATAAGGAGGAGCAACATGGATACGTCACTGGC

P070

Ent C Biosynthesis of Enterobactin Siderophore
(Reverse - GoldenBrick sequence)

53 nt

76°C

GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATGCAATCCAAAAACGTTCAAC

P071

Ent D Biosynthesis of Enterobactin Siderophore
(Forward - GoldenBrick sequence)

70 nt

83°C

GAATTCGGTCTCTTCCACACCCCGAAGAACAGCAAAGGCGGACGAACAATGGTCGATATGAAAACTACGC

P072

Ent D Biosynthesis of Enterobactin Siderophore
(Reverse - GoldenBrick sequence)

47 nt

79°C

GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATCGTGTTGGCACAGC

P073

Ent E Biosynthesis of Enterobactin Siderophore
(Forward - GoldenBrick sequence)

71 nt

80°C

GAATTCGGTCTCTGAACGAGCTAAAAGTAGCGCCCAATATAAGGAGGTCAAAATGAGCATTCCATTCACCC

P074

Ent E Biosynthesis of Enterobactin Siderophore
(Reverse - GoldenBrick sequence)

43 nt

81°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTCAGGCTGATGCGCG

P075

Ent F Biosynthesis of Enterobactin Siderophore
(Forward - GoldenBrick sequence)

68 nt

75°C

GAATTCGGTCTCTGAACACATCTATATTTATCTTAAGGAGTACAATCATGAGCCAGCATTTACCTTTG

P076

Ent F Biosynthesis of Enterobactin Siderophore
(Reverse - GoldenBrick sequence)

47 nt

78°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTACCTGTTTAGCGTTGCG

P077

RBS sfGFP - Plasmid 3
(Forward - GoldenBrick sequence)

65 nt

76°C

CGTGAATTCGGTCTCTGTTCTGAAAGAACTATATCTACAAAATCCATATGCGTAAAGGCGAAGAG

P078

RBS sfGFP - Plasmid 3
(Reverse - GoldenBrick sequence)

58 nt

75°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGTACAGTTCATCCATACCATG

P079

Promoteur Lac + Lac Operator
(Forward - GoldenBrick sequence)

93 nt

82°C

GAATTCGGTCTCAGCCTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCACAGAGTTCAGAGACCAC

P080

Promoteur Lac + Lac Operator
(Reverse - GoldenBrick sequence)

93 nt

82°C

GTGGTCTCTGAACTCTGTGCTCAGTATCTTGTTATCCGCTCACAATGTCAATTGTTATCCGCTCACAATTCTCTAGAGGCTGAGACCGAATTC

P081

RBS positive control - Plasmid 2
(Forward - GoldenBrick sequence)

46 nt

77°C

CGGTCTCTGTGATGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA

P082

RBS positive control - Plasmid 2
(Reverse - GoldenBrick sequence)

46 nt

77°C

TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCATCACAGAGACCG

P083

Lac I - Plasmid 1/2
(Forward - GoldenBrick sequence)

40 nt

72°C

GAATTCGGTCTCTCTAGATGGTGAATGTGAAACCAGTAAC

P084

Lac I - Plasmid 1/2
(Reverse - GoldenBrick sequence)

56 nt

76°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTAAGCTACTAAAGCGTAGTTTTCG

Retrieved from "http://2013.igem.org/Team:Evry/Primers/vf"