Team:Paris Saclay/Notebook/August/23

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Revision as of 22:41, 11 September 2013

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Contents

Notebook : August 23

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3

1 - Electrophoresis to check the digestion of Bba_J04450 by EcoRI/PstI

]]
  • Well 1 : 6µL DNA Ladder
  • Well 2 : 5µL of Bba_J04450 digested by EcoRI/PstI+1µl of 6X loading dye
  • Gel : 1%

Expect sizes :

  • PSB3K3 : 2750 bp

We can see anything. The digestion did work.

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