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Team:Paris Saclay/Notebook/August/29
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Xiaojing (Talk | contribs)
(Created page with "{{Team:Paris_Saclay/incl_debut_generique}} ='''Notebook : August 29'''= =='''summary'''== *We got clonies on ligation promoter fnr(activator)nirB plus RBS_LacZ+Term_PSB1C3 ...")
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(Created page with "{{Team:Paris_Saclay/incl_debut_generique}} ='''Notebook : August 29'''= =='''summary'''== *We got clonies on ligation promoter fnr(activator)nirB plus RBS_LacZ+Term_PSB1C3 ...")
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Revision as of 16:05, 12 September 2013
Notebook : August 29
summary
- We got clonies on ligation promoter fnr(activator)nirB plus RBS_LacZ+Term_PSB1C3
promoter fnr(activator)nirB plus RBS_AmilCP+Term_PSB1C3 promoter fnr(repressor) plus RBS_LacZ+Term_PSB1C3 promoter fnr(repressor) plus RBS_AmilCP+Term_PSB1C3t so we do Colony PCR for it
- We didn't get clonies on Gibson so we do a gel electrophoresis of parts of Gibson assembly to verify the size
- Digestion Dnp1 purification of the the PSB1C3 clean for Gibson and electrophoresis of it .
lab work
- A.aero/anaerobic regulation system
Xiaojing
- Digestion for PSB3K3
| DNA | 9µl |
| Ecoil I | 2µl |
| PST I | 2µl |
| H2O | 5µl |
| buffer | 2µl |
| total | 20µl |
- Digestion for promoter fnr(repressor)
| DNA | 5µl |
| Spe I | 2µl |
| PST I | 2µl |
| H2O | 9µl |
| buffer | 2µl |
| total | 20µl |
