Team:TU-Munich/Results/Recombinant

From 2013.igem.org

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==Characterization of recombinant effector proteins==
==Characterization of recombinant effector proteins==
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Text. See [2013.igem.org/Team:TU-Munich/Notebook/Methods proteinbiochemical methods] for further informatons.
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Text. See [http://www.2013.igem.org/Team:TU-Munich/Notebook/Methods proteinbiochemical methods] for further informatons.
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==Eryhtromycin Esterase==
==Eryhtromycin Esterase==
[[File:TUM13 Animation_EreB.gif|thumb|right|320px| Figure 3:]]
[[File:TUM13 Animation_EreB.gif|thumb|right|320px| Figure 3:]]

Revision as of 20:32, 14 September 2013


Text

Characterization of recombinant effector proteins

Text. See [http://www.2013.igem.org/Team:TU-Munich/Notebook/Methods proteinbiochemical methods] for further informatons.

Table 1:

Investigated Proteins

Protein BioBrick RFC Affinity tag Size [kDa] Disulphid bridges comment
Eryhtromycin Esterase <partinfo>BBa_E0040</partinfo> RFC25 c-term. Streptag II
Laccase <partinfo>BBa_E0040</partinfo> RFC25 c-term. Streptag II
Nano Luciferase <partinfo>BBa_E0040</partinfo> RFC25 c-term. Streptag II
XylE


Eryhtromycin Esterase

File:TUM13 Animation EreB.gif
Figure 3:

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization

Laccase

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization

Nano Luciferase

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization



XylE

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization




DDT-Dehydrochlorinase

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization




SpyCatcher & SpyTag

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization

References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.
  2. http://udel.edu/~gshriver/pdf/Pimenteletal1997.pdf Pmentel et al., 1997 Pimentel, D., Wilson, C., McCullum, C., Huang, R., Dwen, P., Flack, J. Tran, Q., Saltman, T., Cliff, T. (1997). Economic and environmental benefits of biodiversity. BioScience, Vol. 47, No. 11., pp. 747-757.



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