Team:Paris Saclay/Notebook/August/1

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=='''Lab work'''==
=='''Lab work'''==
-
 
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''===
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''===
-
===='''1 - Gibson assembly.'''====
 
-
Andy,Xiaojing
 
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* BphR2_part1, BphR2_part2 and plasmid PSB1C3
+
Objectif : obtaining FNR and BphR2 proteins.
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* FNR_part1, FNR part1 and plasmid PSB1C3
+
-
* RBS_FNR part1, FNR_part2 and plasmid PSB1C3
+
 +
===='''1 - Gel purification of PCR products : BphR2 Part I, BphR2, Part II, FNR Part I, FNR Part II, RBS-FNR Part I, PSB1C3 '''====
 +
XiaoJing
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Sample Volume:
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Protocol : [[Team:Paris_Saclay/Protocols/Gel purification|Gel purification]]
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{|
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| style="width:700px;border:1px solid black;vertical-align:top;" |
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Nanodrop :
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*Tube 1 : BphR2 part1(1ul), BphR2 part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
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* BphR2 Part I : 44 ng/µL
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*Tube 2 : FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
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* BphR2 Part II : 64 ng/µL
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*Tube 3 : RBS_FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
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* FNR Part I : 147 ng/µL
 +
* FNR Part II : 140 ng/µL
 +
* RBS-FNR Part I : 167 ng/µL
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* PSB1C3 : 159 ng/µL
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{|
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| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
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CONCLUSION !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
|}
|}
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These 3 mixture is incubated at 50°C for up to one hour.
 
 +
===='''2 - Gibson assembly.'''====
 +
 +
Xiaojing
 +
 +
Used quantities :
 +
 +
* BphR2 :
 +
** PSB1C3 : 2µL
 +
** BphR2 Part I : 1µL
 +
** BphR2 Part II : 1µL
 +
** Gibson mix : 15µL
 +
** H2O : 1µL
 +
 +
* FNR :
 +
** PSB1C3 : 2µL
 +
** FNR Part I : 1µL
 +
** FNR Part II : 1µL
 +
** Gisbon mix : 15µL ???????????????
 +
** H2O : 1µL
 +
 +
* RBS-FNR :
 +
** PSB1C3 : 2µL
 +
** RBS-FNR Part I : 1µL
 +
** FNR Part II : 1µL
 +
** Gibson mix : 15µL ????????????
 +
** H2O : 1µL
 +
 +
We let these mix at 50°C during 1h.
{{Team:Paris_Saclay/incl_fin}}
{{Team:Paris_Saclay/incl_fin}}

Revision as of 21:09, 15 September 2013

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Contents

Notebook : August 1

Lab work

A - Aerobic/Anaerobic regulation system / B - PCB sensing system

Objectif : obtaining FNR and BphR2 proteins.

1 - Gel purification of PCR products : BphR2 Part I, BphR2, Part II, FNR Part I, FNR Part II, RBS-FNR Part I, PSB1C3

XiaoJing

Protocol : Gel purification

Nanodrop :

  • BphR2 Part I : 44 ng/µL
  • BphR2 Part II : 64 ng/µL
  • FNR Part I : 147 ng/µL
  • FNR Part II : 140 ng/µL
  • RBS-FNR Part I : 167 ng/µL
  • PSB1C3 : 159 ng/µL

CONCLUSION !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

2 - Gibson assembly.

Xiaojing

Used quantities :

  • BphR2 :
    • PSB1C3 : 2µL
    • BphR2 Part I : 1µL
    • BphR2 Part II : 1µL
    • Gibson mix : 15µL
    • H2O : 1µL
  • FNR :
    • PSB1C3 : 2µL
    • FNR Part I : 1µL
    • FNR Part II : 1µL
    • Gisbon mix : 15µL ???????????????
    • H2O : 1µL
  • RBS-FNR :
    • PSB1C3 : 2µL
    • RBS-FNR Part I : 1µL
    • FNR Part II : 1µL
    • Gibson mix : 15µL ????????????
    • H2O : 1µL

We let these mix at 50°C during 1h.


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